Fed-batch pediocin production by Pediococcus acidilactici NRRL B-5627 on whey

• Published in: Biotechnology and applied biochemistry Vol. 42; no. Pt 1; p. 17
• Main Authors: Pérez Guerra, Nelson, Bernárdez, Paula Fajardo, Agrasar, Ana Torrado, López Macías, Cristina, Castro, Lorenzo Pastrana
• Format: Journal Article
• Language: English
• Published: United States 01.08.2005
• Subjects: Animals; Bacteriocins - biosynthesis; Bioreactors - microbiology; Cell Culture Techniques - methods; Cell Proliferation; Milk - metabolism; Pediococcus - growth & development; Pediococcus - metabolism
• ISSN: 0885-4513
• DOI: 10.1042/BA20040146

Cell growth and pediocin production by Pediococcus acidilactici NRRL B-5627 on whey were compared by using batch fermentation and re-alkalized fed-batch fermentation. The batch fermentations were performed on DWG [DW (diluted whey) supplemented with 1% (w/v) glucose], DWYE [DW supplemented with 2% (w/v) yeast extract] and DWGYE (DW supplemented with 1% glucose plus 2% yeast extract) media. The fed-batch culture on DWYE medium was fed with a mixture of concentrated whey (48 g of total sugars/l) supplemented with 2% yeast extract and 400 g/l concentrated glucose. The re-alkalized fed-batch culture was characterized by higher biomass (6.57 g/l) and pediocin [517.6 BU (bacteriocin activity units)/ml] concentrations compared with the batch processes on MRS (de Man, Rogosa and Sharpe) broth (1.76 g/l and 493.2 BU/ml), DW (0.17 g/l and 57.7 BU/ml), DWG (0.14 g/l and 53.6 BU/ml), DWYE (1.43 g/l and 187.6 BU/ml) and DWGYE (1.28 g/l and 167.3 BU/ml) media. A mixed acid fermentation was observed during the growth of P. acidilactici NRRL B-5627 in the fed-batch culture on DWYE medium, and other products (acetic acid and ethanol) in addition to lactic acid accumulated in the medium. Mathematical models were set up to describe fed-batch production of biomass and pediocin by P. acidilactici. The models developed offer a better fit and a more realistic description of the experimental biomass and pediocin production data when compared with the logistic and Luedeking and Piret model.