The fitness costs and benefits of trisomy of each Candida albicans chromosome

• Published in: Genetics (Austin) Vol. 218; no. 2
• Main Authors: Yang, Feng, Todd, Robert T, Selmecki, Anna, Jiang, Yuan-ying, Cao, Yong-bing, Berman, Judith
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 24.06.2021; Genetics Society of America
• Subjects: Aneuploidy; Antifungal Agents - pharmacology; Antifungal Agents - therapeutic use; Candida albicans; Candida albicans - genetics; Candidiasis - drug therapy; Candidiasis - microbiology; Cell walls; Chromosomes; Chromosomes, Fungal - genetics; Collection; Communications; Copy number; Cost benefit analysis; Diploids; Drug Resistance, Fungal - genetics; Fitness; Fungicides; Genetic Fitness; Genetics; Genome, Fungal; Host Microbial Interactions - genetics; Humans; Osmolarity; Oxidative stress; Reproductive fitness; Survival; Trisomy; Yeasts; fitness benefits; monosomy; aneuploidy; fitness costs; trisomy; tetrasomy; antifungal drugs; Candida albicans
• ISSN: 1943-2631; 0016-6731; 1943-2631
• DOI: 10.1093/genetics/iyab056

Abstract Candida albicans is a prevalent human fungal pathogen. Rapid genomic change, due to aneuploidy, is a common mechanism that facilitates survival from multiple types of stresses including the few classes of available antifungal drugs. The stress survival of aneuploids occurs despite the fitness costs attributed to most aneuploids growing under idealized lab conditions. Systematic study of the aneuploid state in C. albicans has been hindered by the lack of a comprehensive collection of aneuploid strains. Here, we describe a collection of diploid C. albicans aneuploid strains, each carrying one extra copy of each chromosome, all from the same genetic background. We tested the fitness of this collection under several physiological conditions including shifts in pH, low glucose, oxidative stress, temperature, high osmolarity, membrane stress, and cell wall stress. We found that most aneuploids, under most conditions, were less fit than their euploid parent, yet there were specific conditions under which specific aneuploid isolates provided a fitness benefit relative to the euploid parent strain. Importantly, this fitness benefit was attributable to the change in the copy number of specific chromosomes. Thus, C. albicans can tolerate aneuploidy of each chromosome and some aneuploids confer improved growth under conditions that the yeast encounters in its host niches.