Characterization of an androgen response element within the promoter of the epididymis-specific murine glutathione peroxidase 5 gene

• Published in: Molecular and cellular endocrinology Vol. 129; no. 1; pp. 33 - 46
• Main Authors: Lareyre, J-J., Claessens, F., Rombauts, W., Dufaure, J-P., Drevet, J.R.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 25.04.1997; Elsevier
• Subjects: Androgens - metabolism; Animals; Anti-oxidant enzyme; Base Sequence; Binding Sites - genetics; Chromosome Mapping; Deoxyribonuclease I; DNA Primers - genetics; Epididymis - metabolism; Gene Expression Regulation, Enzymologic - drug effects; Glutathione Peroxidase - genetics; Hormonal control of gene expression; Humans; Life Sciences; Male; Male genital tract; Mice; Molecular Sequence Data; Mutation; Promoter Regions, Genetic; Receptors, Androgen - genetics; Receptors, Androgen - metabolism; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sperm maturation; Testicular Hormones; DHT, dihydrotestosterone; Sperm maturation; GPX, glutathione peroxidase; GRE, glucocorticoid response element; ARE, androgen response element; Male genital tract; Anti-oxidant enzyme; Hormonal control of gene expression; AR-DBD/A, fusion protein of the DNA-binding domain of the androgen receptor linked to the protein A of Staphylococcus aureus; anti-oxidant enzyme; hormonal control of gene expression; sperm maturation; male genital tract
• ISSN: 0303-7207; 1872-8057; 0303-7207
• DOI: 10.1016/S0303-7207(97)04038-0

We have shown in earlier studies, using a mouse model, that the expression of the glutathione peroxidase 5 protein (GPX5) is restricted to the epididymis and that the accumulation of its corresponding mRNA is hormonally, spatially and temporally regulated throughout postnatal development. We report here, using run-on assays, transient expression experiments as well as gel-shift and footprinting analyses on the findings that at least part of the androgenic control of the GPX5 expression is exerted at the transcriptional level via an androgen response element localized in the distal promoter region of the GPX5 gene. The gpx5 androgen response element (ARE) is found to be consistent with the consensus palindromic steroid-receptor target sequence 5′-AGWACWnnnTGTYCT-3′ but exhibits a quite weak conservation in the left half site. The data presented here further expand the diversity of sequence able to confer androgen responsiveness.