Temperature-response polymer coating for in-tube solid-phase microextraction coupled to high-performance liquid chromatography

• Published in: Talanta (Oxford) Vol. 84; no. 4; pp. 1019 - 1025
• Main Authors: Yu, Qiong-Wei, Ma, Qiao, Feng, Yu-Qi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 30.05.2011; Elsevier
• Subjects: Acrylamides - chemistry; Acrylic Resins; Analytical chemistry; Animals; Capillarity; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Estrogens - analysis; Estrogens - isolation & purification; Exact sciences and technology; In-tube SPME–HPLC; Liquid chromatography; Liquid-phase deposition; Milk - chemistry; Nanocomposites; Nanomaterials; Nanoparticles - chemistry; Nanostructure; Other chromatographic methods; Polarity; Polymers - chemistry; Silicon dioxide; Silicon Dioxide - chemistry; Solid Phase Microextraction - methods; Standard deviation; Temperature; Temperature responsive; Time Factors; Liquid-phase deposition; In-tube SPME–HPLC; Temperature responsive; Performance evaluation; In-tube SPME-HPLC; Temperature; Nanoparticle; Sample; Microanalysis; Estrogen; HPLC chromatography; Polymerization; Polymer; Method; Liquid phase; Coatings; Silica; Solid phase microextraction; Sample preparation; Detection limit; Polarity; Standard deviation; Extraction; Deposition; Milk
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2011.03.003

The silica nanoparticle (SiO2 NP)-deposited capillary fabricated by liquid phase deposition (LPD) was bonded by 3-(triethoxysilyl) propyl methacrylate and then modified with poly(N-isopropylacrylamide) (PNIPAAm) by polymerization. The resulting PNIPAAm modified SiO2 NP-deposited capillary was applied to in-tube solid-phase microextraction coupled to high-performance liquid chromatography (in-tube SPME–HPLC). To investigate the extraction performance of the prepared capillary, diethylstilbestrol (DES) with moderate polarity was selected as the model analyte. Results demonstrate that PNIPAAm modified SiO2 NP-deposited capillary exhibited obvious temperature responsive character. Finally, the PNIPAAm modified SiO2 NP-deposited capillary was applied to the analysis of three synthetical estrogens from milk samples. The detection limit of the method was found to be in the range 1.2–2.2ng/g, and recovery was 71.7–98.9% with relative standard deviations in the range of 2.8–12.6%.