Inhibition of Adipocyte Differentiation by Anthocyanins Isolated from the Fruit of Vitis coignetiae Pulliat is Associated with the Activation of AMPK Signaling Pathway

• Published in: Toxicological research (Seoul) Vol. 34; no. 1; pp. 13 - 21
• Main Authors: Han, Min Ho, Kim, Hong Jae, Jeong, Jin-Woo, Park, Cheol, Kim, Byung Woo, Choi, Yung Hyun
• Format: Journal Article
• Language: English
• Published: Singapore 한국독성학회 01.01.2018; Springer Singapore; Korean Society of Toxicology
• Subjects: Biomedicine; Original; Original Article; Pharmacology/Toxicology; 예방의학; 3T3-L1 preadipocytes; Anthocyanins; AMPK; Vitis coignetiae pulliat; Adipogenesis; pulliat
• ISSN: 1976-8257; 2234-2753
• DOI: 10.5487/TR.2018.34.1.013

Anthocyanins are naturally occurring water-soluble polyphenolic pigments in plants that have been shown to protect against cardiovascular diseases, and certain cancers, as well as other chronic human disorders. However, the anti-obesity effects of anthocyanins are not fully understood. In this study, we investigated the effects of anthocyanins isolated from the fruit of Vitis coignetiae Pulliat on the adipogenesis of 3T3-L1 preadipocytes. Our data indicated that anthocyanins attenuated the terminal differentiation of 3T3-L1 preadipocytes, as confirmed by a decrease in the number of lipid droplets, lipid content, and triglyceride production. During this process, anthocyanins effectively enhanced the activation of the AMP-activated protein kinase (AMPK); however, this phenomenon was inhibited by the co-treatment of compound C, an inhibitor of AMPK. Anthocyanins also inhibited the expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor-γ, CCAAT/enhancer-binding protein a and b, and sterol regulatory element-binding protein-1c. In addition, anthocyanins were found to potently inhibit the expression of adipocyte-specific genes, including adipocyte fatty acid-binding protein, leptin, and fatty acid synthase. These results indicate that anthocyanins have potent anti-obesity effects due to the inhibition of adipocyte differentiation and adipogenesis, and thus may have applications as a potential source for an anti-obesity functional food agent.