Antigenic structure of the capsid protein of rabbit haemorrhagic disease virus
JL Martinez-Torrecuadrada, E Cortes, C Vela, JP Langeveld, RH Meloen, K Dalsgaard, WD Hamilton and JI Casal Immunologia y Genetica Applicada SA, Madrid, Spain. Rabbit haemorrhagic disease virus (RHDV) causes an important disease in rabbits. The virus capsid is composed of a single 60 kDa protein. Th...
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Published in | Journal of general virology Vol. 79; no. 8; pp. 1901 - 1909 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Soc General Microbiol
01.08.1998
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Subjects | |
Online Access | Get full text |
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Summary: | JL Martinez-Torrecuadrada, E Cortes, C Vela, JP Langeveld, RH Meloen, K Dalsgaard, WD Hamilton and JI Casal
Immunologia y Genetica Applicada SA, Madrid, Spain.
Rabbit haemorrhagic disease virus (RHDV) causes an important disease in
rabbits. The virus capsid is composed of a single 60 kDa protein. The
capsid protein gene was cloned in Escherichia coli using the pET3 system,
and the antigenic structure of RHDV VP60 was dissected using 11 monoclonal
antibodies (MAbs) and 12 overlapping fragments of the protein expressed in
E. coli. Two antigenic regions were found. Ten out of the 11 MAbs
recognized different discontinuous epitopes in the most immunodominant
region of the viral capsid. This domain was located between residues 31 and
250 of the VP60 N terminus. The other MAb revealed the presence of an
antigenic site within 102 aa of the C terminus. This MAb did not recognize
the major cleavage product of the full-length 60 kDa protein. These results
indicate that, in contrast to other caliciviruses such as Norwalk virus
(NV), the 36 kDa cleavage product probably forms the N-terminal region of
VP60. However, as in NV, the cleavage region appears to be the most
immunodominant region. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-1317 1465-2099 |
DOI: | 10.1099/0022-1317-79-8-1901 |