Structure and Chromosomal Localization of a Human Water Channel (AQP3) Gene

• Published in: Genomics (San Diego, Calif.) Vol. 27; no. 2; pp. 352 - 354
• Main Authors: Ishibashi, Kenichi, Sasaki, Sei, Saito, Fumiko, Ikeuchi, Tatsuro, Marumo, Fumiaki
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 20.05.1995; Elsevier
• Subjects: Amino Acid Sequence; Animals; Aquaporin 3; Aquaporins; Base Sequence; Biological and medical sciences; Chromosome Mapping; Chromosomes, Human, Pair 7; DNA, Complementary - chemistry; Fundamental and applied biological sciences. Psychology; Genes. Genome; Humans; In Situ Hybridization, Fluorescence; Ion Channels - genetics; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Rats; Genetic mapping; Water; Human; Complementary DNA; Gene; Nucleotide sequence; Homology; C7-Chromosome; Gene expression; Carrier protein
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1995.1055

A cDNA encoding rat AQP3, a water channel and a member of the MIP family, that is expressed predominantly in kidney medulla and colon was cloned recently. To determine the structure, tissue distribution, and chromosomal localization of the human AQP3 gene, we screened a human kidney cDNA library with rat AQP3 probe and isolated a cDNA coding for human AQP3 protein. The deduced amino acid sequence of human AQP3 was 91% identical to rat AQP3. Human AQP3 mRNA was expressed in colon, kidney, liver, pancreas, lung, peripheral leukocytes, spleen, and prostate. The human AQP3 gene was mapped to 7q36.2-q36.3 by chromosome fluorescence in situ hybridization.