Structure of the Human Myelin/Oligodendrocyte Glycoprotein Gene and Multiple Alternative Spliced Isoforms

• Published in: Genomics (San Diego, Calif.) Vol. 29; no. 2; pp. 345 - 352
• Main Authors: PHAM-DINH, DANIELLE, DELLAGASPERA, BRUNO, KERLERODEROSBO, NICOLE, DAUTIGNY, ANDRÉ
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 20.09.1995; Elsevier
• Subjects: Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Blotting, Southern; Brain - metabolism; Exons; Fundamental and applied biological sciences. Psychology; Genes. Genome; Genetic Variation; Humans; Introns; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Myelin Proteins; Myelin-Associated Glycoprotein - biosynthesis; Myelin-Associated Glycoprotein - chemistry; Myelin-Associated Glycoprotein - genetics; Myelin-Oligodendrocyte Glycoprotein; Nerve Tissue Proteins - biosynthesis; Nerve Tissue Proteins - genetics; Protein Structure, Secondary; Restriction Mapping; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Transcription, Genetic; Human; Alternative splicing; Molecular processing; Oligodendrocyte; Nucleotide sequence; Myelin; Central nervous system; Chromosome DNA; Gene organization; Glycoproteins; Lipoprotein
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1995.9995

Myelin/oligodendrocyte glycoprotein (MOG), a specific component of the central nervous system localized on the outermost lamellae of mature myelin, is a member of the immunoglobulin superfamily. We report here the organization of the humanMOGgene, which spans approximately 17 kb, and the characterization of six MOG mRNA splicing variants. The intron/exon structure of the humanMOGgene confirmed the splicing pattern, supporting the hypothesis that mRNA isoforms could arise by alternative splicing of a single gene. In addition to the eight exons coding for the major MOG isoform, the humanMOGgene also contains, in the 3′ region, a previously unknown alternatively spliced coding exon, VIA. Alternative utilization of two acceptor splicing sites for exon VIII could produce two different C-termini. The nucleotide sequences presented here may be a useful tool to study further possible involvement of theMOGgene in hereditary neurological disorders.