Alteromonas addita sp. nov

• Published in: International journal of systematic and evolutionary microbiology Vol. 55; no. 3; pp. 1065 - 1068
• Main Authors: Ivanova, Elena P, Bowman, John P, Lysenko, Anatoly M, Zhukova, Natalia V, Gorshkova, Nataliya M, Sergeev, Alexander F, Mikhailov, Valery V
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01.05.2005
• Subjects: Agar - metabolism; Alteromonas - classification; Alteromonas - cytology; Alteromonas - isolation & purification; Alteromonas addita; Alteromonas infernus; Alteromonas macleodii; Base Composition; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Gelatin - metabolism; Genes, rRNA; Gentian Violet; Growth Inhibitors - pharmacology; Hemolysis; Hydrogen-Ion Concentration; Japan; Molecular Sequence Data; Movement; Nucleic Acid Hybridization; Pacific Ocean; Phenazines; Phospholipids - analysis; Phylogeny; Pigments, Biological; Polysorbates - metabolism; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; Sodium Chloride - pharmacology; Temperature; Water Microbiology; Japan; Pacific Ocean
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63521-0

1 Swinburne University of Technology, PO Box 218, Hawthorn, Victoria 3122, Australia 2 Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100 Let Vladivostoku 159, Russian Federation 3 School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tasmania 7001, Australia 4 S. N. Winogradsky Institute of Microbiology of the Russian Academy of Sciences, 117312 Moscow, Russian Federation 5 Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Sciences, 690041, Vladivostok, Russian Federation 6 V. I. Il'ichev Pacific Oceanological Institute of the Far-Eastern Branch of the Russian Academy of Sciences, Baltiiskaya Str. 43, 690017, Vladivostok, Russian Federation Correspondence Elena P. Ivanova eivanova{at}swin.edu.au On the basis of phenotypic, genotypic characteristics and analysis of 16S rRNA gene sequences, a novel species belonging to the genus Alteromonas is described. A non-pigmented, motile, Gram-negative bacterium designated R10SW13 T was isolated from sea water samples collected in Chazhma Bay (Sea of Japan, Pacific Ocean). The novel organism mainly grew between 4 °C and 37 °C, was neutrophilic and slightly halophilic, tolerating up to 10 % NaCl. Strain R10SW13 T was haemolytic and was able to degrade starch and Tween 80 and to degrade gelatin and agar weakly, but did not degrade casein. Phosphatidylethanolamine (44·3±0·9 %) and phosphatidylglycerol (55·7±0·9 %) were the predominant phospholipids. The major fatty acids formed were typical for the genus Alteromonas , including 16 : 0, 16 : 1 -7 and 18 : 1 -7. The G+C content of the DNA was 43·4 mol%. DNA–DNA hybridization experiments showed 38–53 % binding with the DNAs of type strains of phylogenetically related species of the genus Alteromonas , namely: Alteromonas macleodii , Alteromonas marina , Alteromonas stellipolaris , Alteromonas litorea , ‘ Alteromonas macleodii subsp. fijiensis ’ and ‘ Alteromonas infernus ’. Based on these results, a novel species, Alteromonas addita sp. nov., is proposed, with strain R10SW13 T (=KMM 3600 T =KCTC 12195 T =LMG 22532 T ) as the type strain. Abbreviations: FAME, fatty acid methyl ester The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Alteromonas addita R10SW13 T is AY682202 . DNA G+C contents and DNA–DNA relatedness values for Alteromonas species including A. addita are available as supplementary material in IJSEM Online.