Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing

• Published in: FEMS microbiology ecology Vol. 92; no. 8; p. 1
• Main Authors: Festa, Sabrina, Macchi, Marianela, Cortés, Federico, Morelli, Irma S., Coppotelli, Bibiana M.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.08.2016
• Subjects: Actinomycetales - growth & development; Actinomycetales - metabolism; Aliphatic hydrocarbons; Aromatic hydrocarbons; Base Sequence; Biodegradation, Environmental; Bioremediation; Burkholderiaceae - growth & development; Burkholderiaceae - metabolism; Ecology; Environmental Pollution - analysis; Genetic aspects; Genome, Bacterial - genetics; Microbiology; Microbiota; Phenanthrene; Phenanthrenes - metabolism; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - metabolism; Rhizobiaceae - growth & development; Rhizobiaceae - metabolism; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil; Soil contamination; Soil ecology; Soil Microbiology; Soil Pollutants - metabolism; Sphingomonadaceae - growth & development; Sphingomonadaceae - metabolism; Argentina; pyrosequencing; bioaugmentation; inoculant establishment; community diversity; sp. AM genome; soil microbiome; Sphingobium sp. AM genome
• ISSN: 1574-6941; 0168-6496; 1574-6941
• DOI: 10.1093/femsec/fiw125

The effect of bioaugmentation with Sphingobium sp. AM strain on different soils microbiomes, pristine soil (PS), chronically contaminated soil (IPK) and recently contaminated soil (Phe) and their implications in bioremediation efficiency was studied by focusing on the ecology that drives bacterial communities in response to inoculation. AM strain draft genome codifies genes for metabolism of aromatic and aliphatic hydrocarbons. In Phe, the inoculation improved the elimination of phenanthrene during the whole treatment, whereas in IPK no improvement of degradation of any PAH was observed. Through the pyrosequencing analysis, we observed that inoculation managed to increase the richness and diversity in both contaminated microbiomes, therefore, independently of PAH degradation improvement, we observed clues of inoculant establishment, suggesting it may use other resources to survive. On the other hand, the inoculation did not influence the bacterial community of PS. On both contaminated microbiomes, incubation conditions produced a sharp increase on Actinomycetales and Sphingomonadales orders, while inoculation caused a relative decline of Actinomycetales. Inoculation of most diverse microbiomes, PS and Phe, produced a coupled increase of Sphingomonadales, Burkholderiales and Rhizobiales orders, although it may exist a synergy between those genera; our results suggest that this would not be directly related to PAH degradation. The effect of a bioaugmentation technique on different soils microbiomes showed that inoculation managed to increase the richness and diversity producing a beneficial effect on soil ecology regardless of favouring the process of bioremediation. Graphical Abstract Figure. The effect of a bioaugmentation technique on different soils microbiomes showed that inoculation managed to increase the richness and diversity producing a beneficial effect on soil ecology regardless of favouring the process of bioremediation.