Interaction of heterotrimeric G13 protein with an A-kinase-anchoring protein 110 (AKAP110) mediates cAMP-independent PKA activation

• Published in: Current biology Vol. 11; no. 21; pp. 1686 - 1690
• Main Authors: Niu, Jiaxin, Vaiskunaite, Rita, Suzuki, Nobuchika, Kozasa, Tohru, Carr, Daniel W., Dulin, Nickolai, Voyno-Yasenetskaya, Tatyana A.
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 30.10.2001
• Subjects: A Kinase Anchor Proteins; Adaptor Proteins, Signal Transducing; Carrier Proteins - metabolism; Cells, Cultured; Cyclic AMP - metabolism; Cyclic AMP-Dependent Protein Kinases - metabolism; Enzyme Activation; GTP-Binding Protein alpha Subunits, G12-G13; Guanine Nucleotides - metabolism; Heterotrimeric GTP-Binding Proteins - metabolism; Humans; Kidney - cytology; Male; Models, Biological; Protein Binding; Protein Subunits; Two-Hybrid System Techniques
• ISSN: 0960-9822; 1879-0445
• DOI: 10.1016/S0960-9822(01)00530-9

Heterotrimeric G proteins [1] and protein kinase A (PKA) are two important transmitters that transfer signals from a wide variety of cell surface receptors to generate physiological responses. The established mechanism of PKA activation involves the activation of the Gs-cAMP pathway [2]. Binding of cAMP to the regulatory subunit of PKA (rPKA) leads to a release and subsequent activation of a catalytic subunit of PKA (cPKA). Here, we report a novel mechanism of PKA stimulation that does not require cAMP. Using yeast two-hybrid screening, we found that the α subunit of G13 protein interacted with a member of the PKA-anchoring protein family, AKAP110. Using in vitro binding and coimmunoprecipitation assays, we have shown that only activated Gα13 binds to AKAP110, suggesting a potential role for AKAP110 as a Gα subunit effector protein. Importantly, Gα13, AKAP110, rPKA, and cPKA can form a complex, as shown by coimmunoprecipitation. By characterizing the functional significance of the Gα13-AKAP110 interaction, we have found that Gα13 induced release of the cPKA from the AKAP110-rPKA complex, resulting in a cAMP-independent PKA activation. Finally, AKAP110 significantly potentiated Gα13-induced activation of PKA. Thus, AKAP110 provides a link between heterotrimeric G proteins and cAMP-independent activation of PKA.