Nucleation and the Kinetics of Microtubule Assembly

A kinetic model was developed for the purpose of interpreting scanning calorimetric data of microtubule assembly. The model consists of two steps. The first step is a highly exothermic nucleation with a strong dependence upon temperature and concentration. The second step is the elongation of the nu...

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Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 77; no. 12; pp. 7302 - 7305
Main Authors Jackson, Meyer B., Berkowitz, Steven A.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.12.1980
National Acad Sciences
Subjects
Activation energy
Calorimetry
Computers
Dimers
Heat
Heat measurement
Kinetics
Microtubules
Microtubules - ultrastructure
Models, Biological
Nucleation
Polymerization
Polymers
Protein Binding
Temperature dependence
Thermodynamics
Tubulin - metabolism
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Summary:A kinetic model was developed for the purpose of interpreting scanning calorimetric data of microtubule assembly. The model consists of two steps. The first step is a highly exothermic nucleation with a strong dependence upon temperature and concentration. The second step is the elongation of the nuclei or microtubules by the addition of tubulin dimer to growing ends. Computer fits to the data provided the values of the parameters of the model. The model successfully simulated various experiments.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.77.12.7302