Deletion analysis of the tobacco Nii1 promoter in Arabidopsis thaliana

• Published in: Plant science (Limerick) Vol. 139; no. 1; pp. 71 - 82
• Main Authors: Dorbe, Marie-France, Truong, Hoai-Nam, Crété, Patrice, Daniel-Vedele, Françoise
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 1998; Elsevier Science; Elsevier
• Subjects: Arabidopsis thaliana; beta-glucuronidase; Biological and medical sciences; Deletions; Fundamental and applied biological sciences. Psychology; genetic regulation; genetic transformation; Genetics; leaves; Life Sciences; luciferase; Metabolism; Molecular and cellular biology; Molecular genetics; molecular sequence data; Nicotiana tabacum; Nii promoter; Nitrate; nitrate reductase; Nitrogen metabolism; nucleotide sequences; Plant physiology and development; Plants genetics; promoter regions; recombinant DNA; Regulation; Reporter gene; reporter genes; transcription (genetics); Transcription. Transcription factor. Splicing. Rna processing; transgenic plants; Regulation; Reporter gene; Nitrate; Nii promoter; Deletions; β-Glucuronidase; Enzyme; Induction; Transcription promoter; Luciferase; Nitrates; Gene expression; Arabidopsis thaliana; Regulation(control); Enzymatic activity; Gene; Cruciferae; Ferredoxin-nitrite reductase; Dicotyledones; Glycosidases; Angiospermae; Deletion; Hydrolases; Regulatory sequence; Spermatophyta; Transgenic plant; Oxidoreductases; Experimental plant; O-Glycosidases; GENE REPORTEUR
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/S0168-9452(98)00181-2

A 1 kb promoter fragment of the Nii1 gene, encoding a tobacco foliar nitrite reductase, has been fused to the β-glucuronidase ( Gus) or luciferase ( Luc) reporter genes. These constructs were introduced in Arabidopsis thaliana by in planta infiltration. Analysis of transformants shows that GUS or LUC activities are induced by nitrate. Deletions of the Nii1 promoter fused to the Luc gene have been made in order to delineate cis-sequences necessary for nitrate induction. The Nii1, Δ C, Δ M and Δ H constructs ending, respectively at −962, −678, −202 and −76 bp before the putative transcription start of the Nii1 gene were fused transcriptionally to the Luc reporter gene. Analysis of LUC activities shows that nitrate induction occurs in the Δ MLuc construct, so promoter sequences required for nitrate induction of the reporter gene are retained in the proximal 200 bp fragment of the promoter. For the smallest construct, nitrate inducibility is maintained in one transformant. Yet, the low level of LUC activity detected in the other transformants does not allow us to assume that nitrate regulatory elements are still present in the Δ HLuc construct. We will discuss these results in relation to those obtained with deletions of other Nia or Nii promoters by different laboratories.