Dissecting Diagnostic and Management Strategies for Plant Viral Diseases: What Next?

Recent advancements in molecular biology have revolutionized plant disease diagnosis and management. This review focuses on disease diagnosis through serological techniques, isothermal amplification methods, CRISPR-based approaches, and management strategies using RNA-based methods. Exploring high-t...

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Published inAgriculture (Basel) Vol. 14; no. 2; p. 284
Main Authors Devi, B. Megala, Guruprasath, Samyuktha, Balu, Pooraniammal, Chattopadhyay, Anirudha, Thilagar, Siva Sudha, Dhanabalan, Kanaga Vijayan, Choudhary, Manoj, Moparthi, Swarnalatha, Jailani, A. Abdul Kader
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.02.2024
Subjects
Agriculture
Agrochemicals
CRISPR
Diagnosis
disease diagnosis
Double-stranded RNA
Flowers & plants
Gene expression
Gene sequencing
Gene silencing
Genes
Genetic engineering
Molecular biology
Mutation
Next-generation sequencing
pathogens
pest control
Pesticides
pests
plant disease management
Plant diseases
Plant viruses
precision agriculture
Ribonucleic acid
RNA
RNA interference
RNA-mediated interference
Sustainable agriculture
Viral diseases
Viral infections
virus transmission
Viruses
India
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Summary:Recent advancements in molecular biology have revolutionized plant disease diagnosis and management. This review focuses on disease diagnosis through serological techniques, isothermal amplification methods, CRISPR-based approaches, and management strategies using RNA-based methods. Exploring high-throughput sequencing and RNA interference (RNAi) technologies like host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS), this review delves into their potential. Despite the precision offered by RNAi in pest and pathogen management, challenges such as off-target effects and efficient dsRNA delivery persist. This review discusses the significance of these strategies in preventing aphid-mediated plant virus transmission, emphasizing the crucial role of meticulous dsRNA design for effective viral RNA targeting while minimizing harm to plant RNA. Despite acknowledged challenges, including off-target effects and delivery issues, this review underscores the transformative potential of RNA-based strategies in agriculture. Envisaging reduced pesticide dependency and enhanced productivity, these strategies stand as key players in the future of sustainable agriculture.
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ISSN:2077-0472
2077-0472
DOI:10.3390/agriculture14020284