Construction and Characterisation of an Antifungal Recombinant Bacillus thuringiensis with an Expanded Host Spectrum

• Published in: The journal of microbiology Vol. 50; no. 5; pp. 874 - 877
• Main Authors: Liu, Qin, Seoul National University, Seoul, Republic of Korea, Roh, J.Y., Korea National Institute of Health, Cheongwon, Republic of Korea, Wang, Yong, Lanzhou University, Lanzhou, P. R. China, Choi, J.Y., Seoul National University, Seoul, Republic of Korea, Tao, Xue Ying, Seoul National University, Seoul, Republic of Korea, Kim, J.S., Chonbuk National University, Jeonju, Republic of Korea, Je, Y.H., Seoul National University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Microbiological Society of Korea 01.10.2012; Springer Nature B.V; 한국미생물학회
• Subjects: Agricultural biotechnology; Agriculture; Animals; Antibiosis; antifungal activity; BACILLUS THURINGIENSIS; Bacillus thuringiensis - genetics; Bacillus thuringiensis - isolation & purification; Bacillus thuringiensis - physiology; Barley; Biomedical and Life Sciences; cry1E; Fungi - physiology; Genes; Genetic Engineering; Host Specificity; insecticidal activity; Lepidoptera; Life Sciences; Microbiology; Pest Control; Plant diseases; Plant Diseases - microbiology; Plant Diseases - parasitology; Plasmids; Proteins; Soil Microbiology; Spodoptera - physiology; Spodoptera exigua; Transmission electron microscopy; 생물학; South Korea; insecticidal activity; antifungal activity
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-012-2201-7

A novel antifungal Bacillus thuringiensis strain 19-22, ssp. kurstaki (H3a3b3c), was characterised. This strain included cry1Aa, cry1Ab, cry1Ac, and cry1D, which have high insecticidal activities against lepidopteran larvae other than Spodoptera exigua. To expand the host spectrum, a cry1E gene whose product is active against S. exigua was introduced into the isolate. The transformant successfully expressed the Cry1E protein without any loss of its original antifungal activities. These results indicate that this recombinant strain exhibits dual activities and may be used as an integrated control agent to control plant diseases and insect pests.