Basic Study on the Biological Monitoring for Vanadium—Effects of Vanadium on Vero Cells and the Evaluation of Intracellular Vanadium Contents

• Published in: Biological trace element research Vol. 142; no. 1; pp. 117 - 126
• Main Authors: Mochizuki, Mariko, Kudo, Eiko, Kikuchi, Mitsuho, Takano, Takashi, Taniuchi, Yojiro, Kitamura, Tomoya, Hondo, Ryo, Ueda, Fukiko
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.07.2011; Humana Press Inc; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biomonitoring; Biotechnology; Cell Enlargement; cell growth; Cell Proliferation; Cercopithecus aethiops; Environmental monitoring; Environmental Monitoring - methods; Environmental Pollutants - toxicity; humans; kidneys; Life Sciences; Lithium - metabolism; Monitoring systems; monkeys; Nutrition; Oncology; Pollutants; sodium; Sodium - metabolism; Toxicity; Toxicity Tests; Trace elements; Vanadium; Vanadium - metabolism; Vanadium - toxicity; Vero Cells; washing; ICP-AES; Vanadate; Vero cell; Intracellular content; Vanadium; Biological monitoring; Uptake
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-010-8741-y

A high concentration of vanadium (V) has toxic effects on human and animals and is one of environmental pollutants. In the present study, we have conducted a fundamental study using cultured Vero cells from monkey kidney for the future environmental monitoring. Orthovanadate (VAN), one of V compounds, of 10−10 and 10−8 M did not affect the cell growth although the higher concentration of above 10−6 M VAN inhibited the cell growth accompanied with the decrease in cell numbers and morphological changes. Given that the washing method with ice-cold Li is also effective for determination of the cellular Na content, we used this method for the determination of the V content of the Vero cells. The V distributions in Vero cell; in the 10−3 M VAN solution, extracellular and intracellular were obtained as 1:0.564:0.036 and 1:0.662:0.098 at 60 and 120 min after the treatment of VAN. The intracellular V content was 10% of the applied concentration of VAN. Consequently, it was suggested that V concentration of 10−7 and 10−6 M in the tissue and environment, respectively, might become the threshold concentration; a criterion of the environmental contamination when we carry out environmental monitoring.