Deletion of a Stay-Green Gene Associates with Adaptive Selection in Brassica napus

• Published in: Molecular plant Vol. 9; no. 12; pp. 1559 - 1569
• Main Authors: Qian, Lunwen, Voss-Fels, Kai, Cui, Yixin, Jan, Habib U., Samans, Birgit, Obermeier, Christian, Qian, Wei, Snowdon, Rod J.
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 05.12.2016
• Subjects: allopolyploidy; artificial selection; Brassica napus; Brassica rapa; breeding; chlorophyll; diploidy; ecotypes; gene deletion; gene expression regulation; genes; genetic background; haplotypes; introgression; light harvesting complex; linkage disequilibrium; lipid content; loci; PAV; photosynthesis; photosynthetic reaction centers; photosystem II; plant growth; presence-absence variation; PSII; rapeseed; seed oils; 保绿; 光合作用; 叶绿素含量; 基因缺失; 捕光复合物; 甘蓝型油菜; 适应性; chlorophyll; presence-absence variation; rapeseed; PAV; photosynthesis; breeding
• ISSN: 1674-2052; 1752-9867; 1752-9867
• DOI: 10.1016/j.molp.2016.10.017

Chlorophyll levels provide important imrormation about plant growth and physiological plasticity in response to changing environments. The stay-green gene NON-YELLOWING 1 （NYE1） is believed to regu-late chlorophyll degradation during senescence, concomitantly affecting the disassembly of the light- harvesting complex and hence indirectly influencing photosynthesis. We identified Brassica napus acces- sions carrying an NYE1 deletion associated with increased chlorophyll content, and with upregulated expression of light-harvesting complex and photosynthetic reaction center （PSI and PSII） genes. Compar- ative analysis of the seed oil content of accessions with related genetic backgrounds revealed that the B. napus NYE1 gene deletion （bnnyel） affected oil accumulation, and linkage disequilibrium signatures suggested that the locus has been subject to artificial selection by breeding in oilseed B. napus forms. Comparative analysis of haplotype diversity groups （haplogroups） between three different ecotypes of the allopolyploid B. napus and its A-subgenome diploid progenitor, Brassica rapa, indicated that introgres- sion of the bnnyel deletion from Asian B. rapa into winter-type B. napus may have simultaneously improved its adaptation to cooler environments experienced by autumn-sown rapeseed.