Circulating Endothelial Microparticle Levels Predict Hemodynamic Severity of Pulmonary Hypertension

• Published in: American journal of respiratory and critical care medicine Vol. 177; no. 11; pp. 1268 - 1275
• Main Authors: Amabile, Nicolas, Heiss, Christian, Real, Wendy May, Minasi, Petros, McGlothlin, Dana, Rame, Eduardo J, Grossman, William, De Marco, Teresa, Yeghiazarians, Yerem
• Format: Journal Article
• Language: English
• Published: New York, NY Am Thoracic Soc 01.06.2008; American Lung Association; American Thoracic Society
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Antigens, CD - blood; Biological and medical sciences; Blood platelets; Blood Pressure - physiology; Cardiac catheterization; Cardiovascular disease; Case-Control Studies; Cohort Studies; Endothelium; Female; Heart; Hemodynamics; Humans; Hypertension, Pulmonary - blood; Hypertension, Pulmonary - etiology; Hypertension, Pulmonary - physiopathology; Hypotheses; Intensive care medicine; Intubation; Leukocytes; Male; Medical sciences; Membrane Glycoproteins - blood; Middle Aged; Patients; Pneumology; Predictive Value of Tests; Pulmonary arteries; Pulmonary hypertension; Pulmonary hypertension. Acute cor pulmonale. Pulmonary embolism. Pulmonary vascular diseases; Severity of Illness Index; Vascular Resistance - physiology; United States > US; San Francisco California; Intensive care; Respiratory disease; Cardiovascular disease; microparticles; Hemodynamics; Pulmonary hypertension; Resuscitation; Endothelium
• ISSN: 1073-449X; 1535-4970; 1535-4970
• DOI: 10.1164/rccm.200710-1458OC

Circulating microparticles (MPs) are submicron membrane fragments shed from damaged or activated vascular cells. Endothelial MPs are a biological marker of dysfunctional endothelium. Vascular remodeling and endothelial dysfunction are involved in pulmonary hypertension (PH). We tested the hypothesis that circulating MPs are increased in patients with PH and that identifiable subgroups of MPs predict the hemodynamic severity of this condition progression. Patients (n = 24; age, 54 +/- 4 yr) undergoing right heart catheterization for precapillary PH without any endothelium-active vasodilator therapy participated in the study. Age- and sex-matched healthy control subjects (n = 20) were included. Endothelial (PECAM(+) [CD31(+)]/ CD41(-), VE-cadherin(+) [CD144(+)], and E-selectin(+) [CD62e(+)]), platelet (CD41(+)), leukocyte-derived (CD45(+)), and annexin V(+) MPs were measured by flow cytometry in platelet-free plasma from venous blood. Levels of circulating endothelial PECAM(+), VE-cadherin(+), E-selectin(+), and leukocyte-derived MPs, but not platelet and annexin V(+) MPs, were increased in subjects with PH compared with control subjects (P < 0.01 each). PECAM(+) and VE-cadherin(+) MP levels significantly correlated with mean pulmonary artery pressure (r = 0.92 and r = 0.87, respectively), pulmonary vascular resistance (r = 0.78 and r = 0.73), and mean right atrial pressure (r = 0.43, and r = 0.46) and correlated inversely with cardiac index (r = -0.59 and r = -0.52). These relationships were not observed for other MP subgroups, and persisted in multivariate analysis after adjustment for confounding factors. In subjects with precapillary PH, levels of circulating endothelial and leukocyte MPs were increased compared with control subjects. In addition, levels of PECAM(+) and VE-cadherin(+), but not E-selectin(+), endothelial MPs predicted hemodynamic severity of the disease.