Genetic diversity of three donkey populations in the Croatian coastal region
Genetic variation in three Croatian donkey populations, Istrian (IS), North Adriatic (NA) and Littoral‐Dinaric (LD), was analysed using eight microsatellite loci and by sequence and SSCP analysis of the proximal portion of the mtDNA D‐loop region. The analysis of microsatellite loci revealed observe...
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Published in | Animal genetics Vol. 33; no. 3; pp. 169 - 177 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford UK
Blackwell Science Ltd
01.06.2002
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Genetic variation in three Croatian donkey populations, Istrian (IS), North Adriatic (NA) and Littoral‐Dinaric (LD), was analysed using eight microsatellite loci and by sequence and SSCP analysis of the proximal portion of the mtDNA D‐loop region. The analysis of microsatellite loci revealed observed heterozygosities in the range of 0.37 (MPZ002 in LD) to 0.85 (AHT21 in LD) and polymorphic information content values in the range of 0.36 (MPZ002 in NA) to 0.78 (AHT21 in LD). The overall probability of exclusion was 0.991. Two populations (IS and NA) were closely related (Fst=0.0034), whereas genetic distances between IS and LD (Fst=0.021) and NA and LD (Fst=0.027) were higher. Using AMOVA, 97.6% of the total genetic variance was portioned within populations, while 2.7% was portioned between the Littoral‐Dinaric population and the Istrian/North Adriatic population group. Sequencing of the proximal part of the mtDNA D‐loop region revealed 36 polymorphic sites representing 19 haplotypes which clustered into three haplotype groups (Y, W, Ws). Only the Y haplotype was found in the IS population which is characterized by a large body size. Haplotypes W and Ws were found in the NA and LD populations which include smaller animals. All three haplotypes were found in the LD population, indicating sporadic migration events from the IS into LD donkey population. |
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Bibliography: | ArticleID:AGE879 istex:6859F827DEFC107C1974220B6D63E7B66A7AD4BC ark:/67375/WNG-JCG54Q17-C ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0268-9146 1365-2052 |
DOI: | 10.1046/j.1365-2052.2002.00879.x |