Structural Characterization of Rice Starch in Rice Cake Modified by Thermus scotoductus 4-α-Glucanotransferase (TSαGTase)
Rice cake was produced with a thermostable 4-α-glucanotransferase from Thermus scotoductus (TSαGTase). Starch molecular fine structure, texture, and retrogradation for the enzymatically prepared rice cake were investigated and compared to those for control rice cake. The amylose content in TSαGTase-...
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Published in | Journal of food science Vol. 72; no. 6; pp. C331 - C336 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Malden, USA
Blackwell Publishing Inc
01.08.2007
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Subjects | |
Online Access | Get full text |
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Summary: | Rice cake was produced with a thermostable 4-α-glucanotransferase from Thermus scotoductus (TSαGTase). Starch molecular fine structure, texture, and retrogradation for the enzymatically prepared rice cake were investigated and compared to those for control rice cake. The amylose content in TSαGTase-treated rice cakes decreased, whereas branched and linear malto-oligosaccharides ranging from maltose to maltoheptaose increased slightly. The average molecular weight of the enzyme-treated rice starch in rice cake decreased as amylopectin macromolecules were cleaved and reorganized into small amylopectin clusters. The number of shorter side chains (degree of polymerization [DP] < 9) increased, whereas the number of longer side chains (DP > 10) decreased through the disproportionation reaction of TSαGTase. After 24 h of storage at 4 °C, the enzyme-treated samples demonstrated significantly lower melting enthalpy of retrograded starch (0.4 mJ/mg) compared to that of the control (1.4 mJ/mg). The results indicated that TSαGTase treatment effectively inhibited starch retrogradation in rice cakes. It is suggested that the reduction of amylose content, the rearrangement of amylopectin, and the production of malto-oligosaccharides caused by TSαGTase treatment are responsible for the ineffective molecular reassociation of rice starch in rice cake. |
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Bibliography: | http://dx.doi.org/10.1111/j.1750-3841.2007.00428.x ark:/67375/WNG-RK5KK1B9-C istex:6ADD5CA99C4E7A9CE53A956FB80715D115F36DFA ArticleID:JFDS428 Authors Seo and Roh contributed equally. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-1147 1750-3841 |
DOI: | 10.1111/j.1750-3841.2007.00428.x |