Effects of Spectral Composition Conversion Film and Plant Growth Regulators on Proliferation of Cymbidium Protocorm Like Body (PLB) Cultured In Vitro

• Published in: Environment control in biology Vol. 48; no. 3; pp. 127 - 132
• Main Authors: HAMADA, Kazutoshi, SHIMASAKI, Kazuhiko, OGATA, Tsuneo, NISHIMURA, Yasuyo, NAKAMURA, Karin, OYAMA-EGAWA, Haruka, YOSHIDA, Katsuhira
• Format: Journal Article
• Language: English
• Published: Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists 2010
• Subjects: C. insigne; Cymbidium finlaysonianum; light quality; orchid; red fluorescent film
• ISSN: 1880-554X; 1883-0986
• DOI: 10.2525/ecb.48.127

We examined the effects of the spectral composition conversion red film on the proliferation of protocorm like bodies (PLBs) and formation of shoots and roots in Cymbidium finlaysonianum and C. insigne in the presence and absence of benzyladenine (BA) and 1-naphthaleneacetic acid (NAA). PLBs were cultured under light provided by a cool white fluorescent light and transmitted through the red film with a 16-h photoperiod for 40 days. The addition of NAA (1 mg/L) and BA (2 mg/L) under the cover of the red film increased the number of PLBs significantly; hence, this is the most effective technique for increasing the number of C. finlaysonianum PLBs. Forty days after culture, the order of increase in the number of C. finlaysonianum PLBs was as follows: Red film+NAA+BA>NAA+BA>Red film>Control. In this study, we demonstrated that the spectral composition conversion film can be used as an alternative growth regulator for PLB proliferation in Cymbidium species.