Characterisation of potato isolates of Dickeya chrysanthemi in Spain by a microtitre system for biovar determination

• Published in: Annals of applied biology Vol. 148; no. 2; pp. 157 - 164
• Main Authors: Palacio-Bielsa, A., Cambra, M.A., López, M.M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.04.2006; Blackwell
• Subjects: 5 and 6; Bacterial plant pathogens; Biological and medical sciences; Biovar 1; Biovar 1, 3, 5 and 6; Dickeya chrysanthemi; ELISA; Erwinia; Fundamental and applied biological sciences. Psychology; inoculation; microtitre; PCR; Pectobacterium; Phytopathology. Animal pests. Plant and forest protection; Solanum tuberosum; Southern Europe; Europe; Spain; Plant production; Biovar 1; Vegetables; Plant pathogen; Biology; Determination; Pectobacterium; Temperate zone; Characterization; Polymerase chain reaction; Potato; Inoculation; Erwinia; 3; Biotype; Bacteria; Isolate; 5 and 6; ELISA; Erwinia; inoculation; microtitre; PCR; Pectobacterium; ELISA assay; Enterobacteriaceae; Applied research
• ISSN: 0003-4746; 1744-7348; 1744-1348
• DOI: 10.1111/j.1744-7348.2006.00045.x

Thirteen Spanish potato isolates of Dickeya chrysanthemi obtained from different cultivars, regions and years have been characterised by biochemical, physiological, serological, molecular and pathogenicity tests. Isolates studied have been classified into biovars 1, 3, 5 and 6 by a microtitre system that correlates with the conventional tests in tubes. This is the first description of the characteristics of D. chrysanthemi isolated from potato in Spain and of the development of a microtitre system for biovar determination.