Lack of chick embryotoxicity after 20 kHz, 1.1 mT magnetic field exposure

• Published in: Bioelectromagnetics Vol. 30; no. 7; pp. 573 - 582
• Main Authors: Nishimura, Izumi, Imai, Setsuo, Negishi, Tadashi
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.10.2009
• Subjects: Animals; Chick Embryo - physiology; Chick Embryo - radiation effects; Chickens; Dose-Response Relationship, Radiation; Electromagnetic Fields; embryogenesis; Embryonic Development - radiation effects; intermediate frequency; Radiation Dosage; retinoic acid; Survival Rate; Tissue Survival - physiology; Tissue Survival - radiation effects
• ISSN: 0197-8462; 1521-186X
• DOI: 10.1002/bem.20513

This investigation was undertaken because biological studies to evaluate the effects of intermediate frequency magnetic fields are insufficient. White Leghorn fertile eggs (60/group) were either exposed to a 20 kHz, 1.1 mT(rms) sinusoidal magnetic field or sham‐exposed during the first 2, 7, or 11 days of embryogenesis. Lower dose exposures at 0.011 and 0.11 mT(rms) for 2 days were also conducted to elucidate possible dose–response relationships. Additional eggs given all‐trans‐retinoic acid, a teratogen, were exposed to the 1.1 mT(rms) magnetic field for the same periods to investigate the modification of embryotoxicity. After exposure, embryos were examined for mortality and developmental abnormalities. Developmental stage, number of somite pairs, and other developmental endpoints were also evaluated. Experiments were triplicated and conducted in a blind fashion. No exposure‐related changes were found in any of the endpoints in intact embryos exposed to1.1 mT(rms) or to the lower doses of 0.11 and 0.011 mT(rms) magnetic fields. Retinoic acid administration produced embryotoxic responses, which were embryonic death and developmental abnormalities, in 40–60% of embryos in the sham‐exposed groups. The magnitude of these responses was not changed significantly by the magnetic field exposures. Under the present experimental conditions, exposure to 20 kHz magnetic field up to 1.1 mT(rms) was not embryotoxic in the chick and did not potentiate the embryotoxic action of retinoic acid. Bioelectromagnetics 30:573–582, 2009. © 2009 Wiley‐Liss, Inc.