p53-dependent regulation of heat shock protein 72
Summary Background p53 is a key regulator of the cellular stress response. p53 modulates the transcription of several genes. Objectives To examine the influence of p53 on expression of heat shock protein 72 (HSP72). Methods Two model systems were used. (i) HSP72 expression was studied by Western blo...
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Published in | British journal of dermatology (1951) Vol. 146; no. 5; pp. 786 - 791 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Science Ltd
01.05.2002
Blackwell Oxford University Press |
Subjects | |
Online Access | Get full text |
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Summary: | Summary Background p53 is a key regulator of the cellular stress response. p53 modulates the transcription of several genes.
Objectives To examine the influence of p53 on expression of heat shock protein 72 (HSP72).
Methods Two model systems were used. (i) HSP72 expression was studied by Western blot on extracts from p53‐proficient or p53‐deficient primary mouse keratinocytes, and (ii) archival human anogenital skin from fibroepithelial polyps, human papillomavirus (HPV) 16/18‐associated lesions or squamous cell carcinomas (SCCs) was subjected to immunostaining for HSP72.
Results Basal HSP72 expression was higher in keratinocytes from p53‐deficient than from p53‐proficient mice. Immunostaining for HSP72 was higher in HPV 16/18 lesions and SCCs, which have reduced p53 protein.
Conclusions p53 status may influence the basal level of HSP72. |
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Bibliography: | istex:B2CDCDC4C635DAA498068BC64D356CB08BF9DD58 ArticleID:BJD4721 ark:/67375/WNG-PH2VXK2D-F ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0007-0963 1365-2133 |
DOI: | 10.1046/j.1365-2133.2002.04721.x |