The quality of human skin xenografts on SCID mice: a noninvasive bioengineering approach
Summary Background Animal models are important tools for studies in skin physiology and pathophysiology. Due to substantial differences in skin characteristics such as thickness and number of adnexa, the results of animal studies cannot always be directly transferred to the human situation. Therefo...
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Published in | British journal of dermatology (1951) Vol. 151; no. 5; pp. 971 - 976 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Science Ltd
01.11.2004
Blackwell Oxford University Press |
Subjects | |
Online Access | Get full text |
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Summary: | Summary
Background Animal models are important tools for studies in skin physiology and pathophysiology. Due to substantial differences in skin characteristics such as thickness and number of adnexa, the results of animal studies cannot always be directly transferred to the human situation. Therefore, transplantation of human skin on to SCID (severe combined immunodeficiency) mice might offer a promising tool to perform studies in viable human skin without the direct need for human volunteers.
Objectives To characterize the physiological and anatomical changes of a human skin transplant on a SCID animal host.
Methods In this study human skin was transplanted on to 32 SCID mice and followed for 6 months. Barrier function was assessed by transepidermal water loss (TEWL; tewametry) and moisture content of the stratum corneum was studied by measurement of electrical capacitance (corneometry).
Results The results showed considerable deviations of TEWL values and skin hydration between the grafts and human skin in vivo. The human skin showed epidermal hyperkeratosis and moderate sclerosis of the corium 4 and 6 months after transplantation on to SCID mice.
Conclusions Our results indicate that human skin does not completely preserve its physiological and morphological properties after transplantation on to SCID mice. Therefore, results from experiments using this model system need to be discussed cautiously. |
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Bibliography: | ArticleID:BJD6191 ark:/67375/WNG-VKW4SHTJ-X istex:08CD4E8E5D7E7D03AE254C1145DF8EC6F219BAEA This study was carried out at both laboratories. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0007-0963 1365-2133 |
DOI: | 10.1111/j.1365-2133.2004.06191.x |