Dihydroxynonene mercapturic acid, a urinary metabolite of 4-hydroxynonenal, as a biomarker of lipid peroxidation

• Published in: BioFactors (Oxford) Vol. 24; no. 1-4; pp. 89 - 96
• Main Authors: Peiro, GÉRaldine, Alary, Jacques, Cravedi, Jean-Pierre, Rathahao, Estelle, Steghens, Jean-Paul, Guéraud, FranÇOise
• Format: Journal Article
• Language: English
• Published: Amsterdam IOS Press 2005; Wiley
• Subjects: 4-hydroxynonenal; Acetylcysteine - analogs & derivatives; Acetylcysteine - urine; Aldehydes - urine; Animals; biomarker; Biomarkers - urine; Bromotrichloromethane - administration & dosage; Chromatography, Liquid; dihydroxynonene mercapturic acid; Dinoprost - analogs & derivatives; Dinoprost - urine; Kinetics; Life Sciences; Lipid Peroxidation; Male; Malondialdehyde - urine; Mass Spectrometry; Oxidative Stress; Rats; Rats, Wistar; urine
• ISSN: 0951-6433; 1872-8081
• DOI: 10.1002/biof.5520240110

The objective of our study was to compare the information obtained through the use of three different urinary biomarkers of lipoperoxidation during the time course of a bromotrichloromethane (BrCCl3) induced oxidative stress in rats. These biomarkers were malondialdehyde (MDA) measured by LC/MS after derivatization, the isoprostane 8‐iso‐PGF2α measured by enzyme immunoassay and 1,4‐dihydroxynonene mercapturic acid (DHN‐MA), the major 4‐hydroxynonenal urinary metabolite [1], measured by LC‐MS. Male Wistar rats received a single dose of 100 μL/kg BrCCl3 per os and lipid peroxidation was estimated every day for a 4‐day‐period after treatment. MDA, 8‐iso‐PGF2α and DHN‐MA significantly increased in response to BrCCl3 treatment for this period of time, and DHN‐MA showed the main increase during the 24–48 h period after treatment.