Overexpression of p28GANK accelerates the metastasis of oesophageal squamous cell carcinoma

• Published in: Molecular medicine reports Vol. 11; no. 6; pp. 4291 - 4296
• Main Authors: TANG, SHANHONG, QIN, JIANPING, LIU, WEIHUI, ZHENG, XIUSHAN, WU, XIAOLING, XU, HUI, QIAO, LIJUAN, FAN, QUANSHUI, ZENG, WEIZHENG, JIANG, MINGDE
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.06.2015; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Adult; Aged; Analysis; Biotechnology; Breast cancer; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Cell Line, Tumor; Cell Movement - genetics; Esophageal cancer; Esophageal Neoplasms - genetics; Esophageal Neoplasms - metabolism; Esophageal Neoplasms - pathology; Esophageal Squamous Cell Carcinoma; Esophagus; Female; Gene Expression; Gene Knockdown Techniques; Genetic aspects; Hospitals; Humans; Immunohistochemistry; invasiveness; Lymph nodes; Lymphatic system; Male; Metastases; Metastasis; Middle Aged; mRNA; Neoplasm Metastasis; Neoplasm Staging; oesophageal squamous cell carcinoma; p28GANK; Polymerase chain reaction; Proteasome Endopeptidase Complex - genetics; Proteasome Endopeptidase Complex - metabolism; Protein expression; Proteins; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; RNA Interference; siRNA; Squamous cell carcinoma; Studies; Thoracic surgery; China; United States > US
• ISSN: 1791-2997; 1791-3004
• DOI: 10.3892/mmr.2015.3286

The present study aimed to evaluate the effects of p28GANK expression on the metastasis of oesophageal squamous cell carcinoma (ESCC) tissues and to investigate its roles in the metastasis of highly invasive and non-invasive ESCC cell lines. Quantitative polymerase chain reaction (qPCR) and immunohistochemical analyses were performed to assess p28GANK mRNA and protein expression in ESCC tissues and to analyse its significance in ESCC metastasis. qPCR and western blot analyses were used to detect p28GANK mRNA and protein expression in highly invasive and non-invasive cell lines. Subsequently, lentivirus-mediated p28GANK short interfering RNA (siRNA) was transfected into highly invasive ESCC cells, and Transwell assays were performed to analyse the effects of p28GANK knockdown on their migration and invasion. The mean expression levels of p28GANK mRNA in the ESCC tissues of patients with metastasis were significantly higher than those in the ESCC specimens from patients without metastasis. p28GANK expression in ESCC tissues was correlated with T-stage, lymph node metastasis and lymphatic invasion. The mRNA and protein expression levels of p28GANK were significantly higher in highly invasive cell lines compared with those of matched, non-invasive cell lines. Lentivirus-mediated siRNA knockdown of p28GANK markedly decreased p28GANK expression in EC109-P and EC9706-P cells and supressed the metastasis of ESCC cells in vitro. In conclusion, p28GANK expression was increased in metastatic ESCC tissues and cells, and p28GANK knockdown decreased the metastatic ability of ESCC cells. These results suggested that p28GANK may be a potential therapeutic marker for ESCC metastasis.