Novel cell-based odorant sensor elements based on insect odorant receptors

• Published in: Biosensors & bioelectronics Vol. 65; pp. 287 - 294
• Main Authors: Mitsuno, Hidefumi, Sakurai, Takeshi, Namiki, Shigehiro, Mitsuhashi, Hiroyuki, Kanzaki, Ryohei
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.03.2015
• Subjects: Alkadienes - analysis; Animals; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biotechnology; Calcium - analysis; Calcium imaging; Cell Line; Chips; Displays; Equipment Design; Fatty Alcohols - analysis; Gene Expression; Humans; Insect odorant receptor; Insect Proteins - genetics; Insect Proteins - metabolism; Insecta - cytology; Insecta - genetics; Insecta - metabolism; Insects; Lab-On-A-Chip Devices; Odorant sensor element; Odorants; Odorants - analysis; Receptors; Receptors, Odorant - genetics; Receptors, Odorant - metabolism; Selectivity; Sensors; Sf21 cell line; Insect odorant receptor; Calcium imaging; Sf21 cell line; Odorant sensor element
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2014.10.026

Development of cell-based odorant sensor elements combined not only high degree of sensitivity and selectivity but also long-term stability is crucial for their practical applications. Here we report the development of a novel cell-based odorant sensor element that sensitively and selectively detects odorants and displays increased fluorescent intensities over a long period of time. Our odorant sensor elements, based on Sf21 cell lines expressing insect odorant receptors, are sensitive to the level of several tens of parts per billion in solution, can selectively distinguish between different types of odorants based on the odorant selectivity intrinsic to the expressed receptors, and have response times of approximately 13s. Specifically, with the use of Sf21 cells and insect odorant receptors, we demonstrated that the established cell lines stably expressing insect odorant receptors are able to detect odorants with consistent responsiveness for at least 2 months, thus exceeding the short life-span normally associated with cell-based sensors. We also demonstrated the development of a compact odorant sensor chip by integrating the established insect cell lines into a microfluidic chip. The methodology we established in this study, in conjunction with the large repertoire of insect odorant receptors, will aid in the development of practical cell-based odorant sensors for various applications, including food administration and health management. •We developed the methodology for cell-based sensor elements with insect odorant receptors (ORs).•We established Sf21 cell lines with insect ORs, Orco, and GCaMP3 as sensor elements.•The ORs determined response characteristics of the sensor elements.•The cell lines exhibited the invariable responsiveness for at least 2 months.•We developed a compact odorant sensor using the cell lines and a microfluidic chip.