Production of fructooligosaccharides by mycelium-bound transfructosylation activity present in Cladosporium cladosporioides and Penicilium sizovae

• Published in: Process biochemistry (1991) Vol. 49; no. 12; pp. 2174 - 2180
• Main Authors: Zambelli, P., Fernandez-Arrojo, L., Romano, D., Santos-Moriano, P., Gimeno-Perez, M., Poveda, A., Gandolfi, R., Fernández-Lobato, M., Molinari, F., Plou, F.J.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.12.2014
• Subjects: Bioactive oligosaccharides; Biochemistry; Blastose; Chromatography; Cladding; Cladosporium cladosporioides; Conversion; Fructooligosaccharides; Hydrolysis; Prebiotics; Purification; Strain; Sucrose; Transfructosylation; Bioactive oligosaccharides; Transfructosylation; Blastose; Fructooligosaccharides; Prebiotics
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2014.09.021

•Several filamentous fungi with transfructosylating activity were isolated from molasses and jams.•The activity was mostly mycelium-bound.•Cladosporium cladosporioides synthesized a mixture of inulin-type FOS, neoFOS and levan-type FOS.•The disaccharide blastose (sucrose isomer) was obtained in high yield. Different filamentous fungi isolated from molasses and jams (kiwi and fig) were screened for fructooligosaccharides (FOS) producing activity. Two strains, identified as Penicilium sizovae (CK1) and Cladosporium cladosporioides (CF215), were selected on the basis of the FOS yield and kestose/nystose ratio. In both strains the activity was mostly mycelium-bound. Starting from 600g/L of sucrose, maximum FOS yield was 184 and 339g/L for P. sizovae and C. cladosporioides, respectively. Interestingly, the highest FOS concentration with C. cladosporioides was reached at 93% sucrose conversion, which indicated a notable transglycosylation to hydrolysis ratio. The main FOS in the reaction mixtures were identified by HPAEC–PAD chromatography. C. cladosporioides synthesized mainly 1-kestose (158g/L), nystose (97g/L), 1F-fructosylnystose (19g/L), 6-kestose (12g/L), neokestose (10g/L) and a disaccharide (34g/L) that after its purification and NMR analysis was identified as blastose [Fru-β(2→6)-Glc]. P. sizovae was very selective for the formation of 1F-FOS (in particular 1-kestose) with minor contribution of neoFOS and negligible of levan-type FOS.