Map-based cloning of a gene sequence encoding a nucleotide-binding domain and a leucine-rich region at the Cre3 nematode resistance locus of wheat

• Published in: Genome Vol. 40; no. 5; pp. 659 - 665
• Main Authors: Lagudah, E.S, Moullet, O, Appels, R
• Format: Journal Article
• Language: English
• Published: Canada 01.10.1997
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; binding proteins; Binding Sites - genetics; Chromosome Mapping; chromosomes; cloning; Cloning, Molecular; complementary DNA; cre3 gene; cre3 locus; gene location; genes; Heterodera avenae; Leucine - genetics; loci; Molecular Sequence Data; Nematoda - pathogenicity; nucleotide sequences; nucleotides; pest resistance; Plant Diseases - genetics; Plant Diseases - parasitology; Plant Proteins - genetics; Plant Roots - parasitology; polypeptides; pseudogenes; Restriction Mapping; roots; Sequence Alignment; Triticum; Triticum - genetics; Triticum - immunology; triticum tauschii
• ISSN: 0831-2796; 1480-3321
• DOI: 10.1139/g97-087

The Cre3 gene confers a high level of resistance to the root endoparasitic nematode Heterodera avenae in wheat. A DNA marker cosegregating with H. avenae resistance was used as an entry point for map-based cloning of a disease resistance gene family at the Cre3 locus. Two related gene sequences have been analysed at the Cre3 locus. One, identified as a cDNA clone, encodes a polypeptide with a nucleotide binding site (NBS) and a leucine-rich region; this member of the disease resistance gene family is expressed in roots. A second Cre3 gene sequence, cloned as genomic DNA, appears to be a pseudogene, with a frame shift caused by a deletion event. These two genes, related to members of the cytoplasmic NBS--leucine rich repeat class of plant disease resistance genes were physically mapped to the distal 0.06 fragment of the long arm of wheat chromosome 2D and cosegregated with nematode resistance.