Identification of SMEK2 as a candidate gene for regulation of responsiveness to dietary cholesterol in rats

We have previously mapped a diet-induced hypercholesterolemia locus (Dihc2) to chromosome 14 in the F2 generation cross of high-responsive exogenous hypercholesterolemia rats and low-responsive BN rats. To identify a causal gene within this locus, we constructed interval-specific congenic lines and...

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Published inJournal of lipid research Vol. 50; no. 1; pp. 41 - 46
Main Authors Asahina, Makoto, Haruyama, Waka, Ichida, Yasuhiro, Sakamoto, Mai, Sato, Masao, Imaizumi, Katsumi
Format Journal Article
LanguageEnglish
Published United States American Society for Biochemistry and Molecular Biology 2009
Subjects
Animals
Base Sequence
Cholesterol - metabolism
Cholesterol, Dietary
Codon, Nonsense
Diet
DNA Mutational Analysis
Hypercholesterolemia - genetics
Lipids - chemistry
Liver - metabolism
Models, Biological
Molecular Sequence Data
Rats
Rats, Transgenic
RNA, Messenger - metabolism
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Summary:We have previously mapped a diet-induced hypercholesterolemia locus (Dihc2) to chromosome 14 in the F2 generation cross of high-responsive exogenous hypercholesterolemia rats and low-responsive BN rats. To identify a causal gene within this locus, we constructed interval-specific congenic lines and carried out expression and sequencing analyses. Here we narrowed Dihc2 to a region including 33 genes and predicted transcripts and identified RGD1309450_predicted, a homologous gene of SMEK2, as a strong candidate for responsiveness to dietary cholesterol. Our finding provides new insights into the pathway underlying the individual responsiveness to dietary cholesterol in vivo.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-2275
1539-7262
DOI:10.1194/jlr.m800135-jlr200