Canine Parvovirus Type 2a (CPV-2a)-Induced Apoptosis in MDCK Involves Both Extrinsic and Intrinsic Pathways

• Published in: Applied biochemistry and biotechnology Vol. 172; no. 1; pp. 497 - 508
• Main Authors: Doley, Juwar, Singh, Lakshya Veer, Kumar, G. Ravi, Sahoo, Aditya Prasad, Saxena, Lovleen, Chaturvedi, Uttara, Saxena, Shikha, Kumar, Rajiv, Singh, Prafull Kumar, Rajmani, R. S, Santra, Lakshman, Palia, S. K, Tiwari, S, Harish, D. R, Kumar, Arvind, Desai, G. S, Gupta, Smita, Gupta, Shishir K, Tiwari, A. K
• Format: Journal Article
• Language: English
• Published: Boston Springer-Verlag 2014; Springer US; Springer; Springer Nature B.V
• Subjects: acute course; Animal diseases; Animals; Apoptosis; Biochemistry; Biological and medical sciences; Biological Transport; Biotechnology; Canine parvovirus; Caspases - metabolism; Cell cycle; Cell Membrane - metabolism; Chemistry; Chemistry and Materials Science; Cytoplasm; Deoxyribonucleic acid; Diploidy; DNA; DNA damage; DNA fragmentation; Dogs; endoplasmic reticulum; Endoplasmic Reticulum - metabolism; Feline panleukopenia virus; Fundamental and applied biological sciences. Psychology; Madin Darby Canine Kidney Cells; Nucleosomes - metabolism; Parvovirus, Canine - physiology; Phosphatidylserines - metabolism; Signal Transduction; Translocation; Tumor Suppressor Protein p53 - metabolism; viruses; Caspase; Apoptotic pathways; CPV-2; MDCK cells; Apoptosis; Parvovirus; Enzyme; Virus; Peptidases; Canine parvovirus; Parvoviridae; Hydrolases; Parvovirinae
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-013-0538-y

The canine parvovirus type 2 (CPV-2) causes an acute disease in dogs. It has been found to induce cell cycle arrest and DNA damage leading to cellular lysis. In this paper, we evaluated the apoptotic potential of the “new CPV-2a” in MDCK cells and elucidated the mechanism of the induction of apoptosis. The exposure of MDCK cells to the virus was found to trigger apoptotic response. Apoptosis was confirmed by phosphatidylserine translocation, DNA fragmentation assays, and cell cycle analysis. Activation of caspases-3, -8, -9, and -12 and decrease in mitochondrial potential in CPV-2a-infected MDCK cells suggested that the CPV-2a-induced apoptosis is caspase dependent involving extrinsic, intrinsic, and endoplasmic reticulum pathways. Increase in p53 and Bax/Bcl2 ratio was also observed in CPV-2a-infected cells.