Na+-sensing quantum dots for cell-based screening of intracellular Na+ concentrations ([Na+]i)

• Published in: Talanta (Oxford) Vol. 85; no. 1; pp. 694 - 700
• Main Authors: Wang, Yuchi, Mao, Hua, Wong, Lid B.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.07.2011; Elsevier
• Subjects: Analytical chemistry; Animals; Bumetanide; Cell Line; Channels; Chemistry; Drug Evaluation, Preclinical - methods; Exact sciences and technology; Homeostasis; HTS drug discovery; Human; Humans; Lidocaine; Myocytes, Cardiac - metabolism; Na+ channel; Nanocomposites; Nanomaterials; Nanostructure; Physiological responses; Quantum Dots; Rats; Screening; Sodium - analysis; Sodium - metabolism; Sodium ionophore; Sodium-Hydrogen Exchangers - physiology; Sodium-Potassium-Chloride Symporters - physiology; HTS drug discovery; Na+ channel; Bumetanide; Lidocaine; Sodium ionophore; Human; Drug; Dynamic response; Rat; Antiporter; Rodentia; channel; Concentration; Permeability; Myocyte; Screening; Vertebrata; Mammalia; Na; Sodium; Membrane; Intracellular; Ionophore
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2011.04.048

We have developed a Na-quantum dot (QD) nanosensor for [Na+]i measurements. Using this Na-QD, we determined the dynamic physiological responses of [Na+]i in nonexcitable human HEK-293F cells and excitable primary rat cardiac myocytes by pharmacologically manipulating the membrane permeability to Na+, the Na–K–2Cl cotransporter, and the Na+/H+ antiporter. These data suggest that the mechanisms of [Na+]i homeostasis can now be elucidated with this novel Na-QD nanosensor. This could have a broad impact on Na+ channel drug discovery.