A DREB-Like Transcription Factor From Maize (Zea mays), ZmDREB4.1, Plays a Negative Role in Plant Growth and Development

The DREB (dehydration-responsive element binding)-type transcription factors are classified into six subgroups, named A-1 to A-6. The members of DREB A-1 and A-2 subgroups have been reported to be involved in response to various abiotic stresses. However, there were only a few genes belonging to A-3...

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Published inFrontiers in plant science Vol. 9; p. 395
Main Authors Li, Shixue, Zhao, Qian, Zhu, Dengyun, Yu, Jingjuan
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 04.04.2018
Subjects
DREB
dwarfism
maize
Plant Science
tobacco
transcriptional activator
ZmDREB4.1
tobacco
DREB
transcriptional activator
maize
ZmDREB4.1
dwarfism
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Summary:The DREB (dehydration-responsive element binding)-type transcription factors are classified into six subgroups, named A-1 to A-6. The members of DREB A-1 and A-2 subgroups have been reported to be involved in response to various abiotic stresses. However, there were only a few genes belonging to A-3 to A-6 subgroups to be reported. In this study, we cloned a DREB A-4 subgroup gene from maize ( ), , and analyzed its characteristics and functions. was expressed in roots, stems, and leaves at very low levels. It was not induced by any biotic or abiotic treatment. ZmDREB4.1 was located in the nucleus, could directly bind to the DRE element and functioned as a transcriptional activator. The constitutive expression of in tobacco ( L.) repressed leaf extension and hypocotyl, petiole and stem elongation. In maize, overexpression of repressed calli growth and regeneration. Further analysis showed that the smaller leaves of transgenic tobacco resulted from inhibition of cell division. The contents of cytokinin and auxin in transgenic leaves were severely decreased. The shorter hypocotyls, stems and petioles of transgenic tobacco were caused by inhibition of cell elongation. The transgenic hypocotyls, stems and petioles contained reduced gibberellin levels. Application of exogenous GA3 rescued the shorter hypocotyls, stems and petioles, but not the smaller leaves. These results demonstrated that plays an important role in the negative regulation of plant growth and development.
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This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science
Edited by: Michael James Considine, University of Western Australia, Australia
Reviewed by: M. Teresa Sanchez-Ballesta, Instituto de Ciencia y Tecnología de Alimentos y Nutrición (ICTAN-CSIC), Spain; Nobutaka Mitsuda, National Institute of Advanced Industrial Science and Technology (AIST), Japan
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2018.00395