Functional Analysis of the PgCesA3 White Spruce Cellulose Synthase Gene Promoter in Secondary Xylem

• Published in: Frontiers in plant science Vol. 10; p. 626
• Main Authors: Sena, Juliana Stival, Lachance, Denis, Duval, Isabelle, Nguyen, Thi Thuy An, Stewart, Don, Mackay, John, Séguin, Armand
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 28.05.2019
• Subjects: cellulose synthase phylogeny; conifer; electrophoretic mobility shift assay; functional analysis; MYB regulatory binding sites; plant genetic transformation; Plant Science; MYB regulatory binding sites; electrophoretic mobility shift assay; functional analysis; plant genetic transformation; cellulose synthase phylogeny; conifer
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2019.00626

Cellulose is an essential structural component of the plant cell wall. Its biosynthesis involves genes encoding cellulose synthase enzymes and a complex transcriptional regulatory network. Three cellulose synthases have been identified in conifers as being potentially involved in secondary cell wall biosynthesis because of their preferential expression in xylem tissues; however, no direct functional association has been made to date. In the present work, we characterized the white spruce [ (Moench) Voss] cellulose synthase gene and 5' regulatory elements. Phylogenetic analysis showed that genes grouped with secondary cell wall-associated Arabidopsis cellulose synthase genes, such as , and . We produced transgenic spruce expressing the GUS reporter gene driven by the promoter. We observed blue staining in differentiating xylem cells from stem and roots, and in foliar guard cells indicating that is clearly involved in secondary cell wall biosynthesis. The promoter region sequence of contained several putative MYB cis-regulatory elements including AC-I like motifs and secondary wall MYB-responsive element (SMRE); however, it lacked SMRE4, 7 and 8 that correspond to the sequences of AC-I, II, and III. Based on these findings and results of previous transient trans-activation assays that identified interactions between the promoter and different MYB transcription factors, we performed electrophoretic mobility shift assays with MYB recombinant proteins and cis-regulatory elements present in the promoter. We found that PgMYB12 bound to a canonical AC-I element identified in the PAL promoter and two AC-I like elements. We hypothesized that the PgMYB12 could regulate in roots based on previous expression results. This functional study of sequences and promoter opens the door for future studies on the interaction between PgMYBs and the regulatory elements.