High-speed simultaneous ion-exclusion/cation-exchange chromatography of anions and cations on a weakly acidic cation-exchange resin column

• Published in: Journal of Chromatography A Vol. 997; no. 1; pp. 219 - 224
• Main Authors: Mori, Masanobu, Tanaka, Kazuhiko, Helaleh, Murad I.H., Xu, Qun, Ikedo, Mikaru, Ogura, Yutaka, Sato, Shinji, Hu, Wenzhi, Hasebe, Kiyoshi, Haddad, Paul R.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 16.05.2003; Elsevier
• Subjects: Analysis methods; Analytical chemistry; Anions - isolation & purification; Applied sciences; Calibration; Cation Exchange Resins; Cations - isolation & purification; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Ion Exchange - methods; Crown Ethers; Earth, ocean, space; Environmental Monitoring; Exact sciences and technology; External geophysics; Hydrogen-Ion Concentration; Inorganic anions; Inorganic cations; Meteorology; Natural water pollution; Other chromatographic methods; Pollution; Quality Control; Reproducibility of Results; Tartaric acid; Tartrates; Water - analysis; Water in the atmosphere (humidity, clouds, evaporation, precipitation); Water treatment and pollution; Inorganic cations; Environmental analysis; Tartaric acid; Water analysis; Inorganic anions; Methacrylate polymer; Sodium Ions; Ammonium; Chemical analysis; Crown compound; Nitrates; Ion exclusion chromatography; Mobile phase; Sulfates; Potassium Ions; Alkaline earth metal Ions; Ion exchange chromatography; Chlorides; Alkali metal Ions; Magnesium Ions; Quantitative analysis; Cationic resin; Phase composition; Calcium Ions; Inorganic cation; Inorganic anion; Conductometry; Surface water; Simultaneous measurement; Water quality; Rain water; Water pollution; River water
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(03)00631-9

The simultaneous ion-exclusion/cation-exchange separation column packed with a polymethacrylate-based weakly acidic cation-exchange resin of 3 μm particle size was used to achieve the simultaneous high-speed separation of anions and cations (Cl −, NO 3 −, SO 4 2−, Na +, K +, NH 4 +, Ca 2+ and Mg 2+) commonly found in environmental samples. The high-speed simultaneous separation is based on a combination of the ion-exclusion mechanism for the anions and the cation-exchange mechanism for cations. The complete separation of the anions and cations was achieved in 5 min by elution with 15 m M tartaric acid–2.5 m M 18-crown-6 at a flow-rate of 1.5 ml/min. Detection limits at S/ N=3 ranged from 0.36 to 0.68 μ M for anions and 0.63–0.99 μ M for cations. This method has been applied to the simultaneous determination of anions and cations in several environmental waters with satisfactory results.