Near-infrared fluorescent probe based on rhodamine derivative for detection of NADH in live cells

• Published in: Methods (San Diego, Calif.) Vol. 204; pp. 22 - 28
• Main Authors: Zhang, Yibin, Arachchige, Dilka Liyana, Olowolagba, Adenike, Luck, Rudy L., Liu, Haiying
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2022
• Subjects: Cellular Imaging; Computation chemistry; Fluorescence; Fluorescent Dyes; HeLa Cells; Humans; NAD; NADH; Near-infrared fluorescent probe; Rhodamines; Cellular Imaging; NADH; Near-infrared fluorescent probe; Computation chemistry
• ISSN: 1046-2023; 1095-9130
• DOI: 10.1016/j.ymeth.2022.03.019

Near-infrared fluorescent probe was prepared for selective detection of reduced nicotinamide adenine dinucleotide (NADH) in live cells. [Display omitted] •A novel fluorescent probe was prepared for sensitive detection of NADH in the near-infrared emission region of 740 nm.•Computation chemistry was used to predict optical properties of the probe in the absence and presence of NADH.•The probe was used to detect NADH concentration changes in HeLa cells caused by glucose treatment and under different pyruvate/lactate ratio levels. A near-infrared fluorescent probe was prepared for selective detection of reduced nicotinamide adenine dinucleotide (NADH) in live cells. The probe turns off the fluorescence with a closed spironolactone switch. However, reduction of the probe by NADH turns on fluorescence at 740 nm. Theoretical calculations suggest a more planar arrangement between the rhodamine and quinoline moieties with increased π-delocalization resulting from reduction.