Modification of the deoxy-myoglobin/carbonmonoxy-myoglobin UV-vis assay for reliable determination of CO-release rates from organometallic carbonyl complexes

The deoxy-myoglobin (deoxy-Mb)/carbonmonoxy-myoglobin (Mb-CO) UV-vis assay is the principal method used for quantifying the rates of CO release from CO-releasing molecules (CO-RMs) that might possess therapeutic benefits. Some issues emerge when the Mb-CO assay is utilized for testing CO-RMs with no...

Full description

Saved in:
Bibliographic Details
Published inDalton transactions : an international journal of inorganic chemistry Vol. 40; no. 21; pp. 5755 - 5761
Main Authors Atkin, Anthony J, Lynam, Jason M, Moulton, Benjamin E, Sawle, Philip, Motterlini, Roberto, Boyle, Nicola M, Pryce, Mary T, Fairlamb, Ian J S
Format Journal Article
LanguageEnglish
Published England 01.01.2011
Subjects
Assaying
Carbon monoxide
Carbon Monoxide - analysis
Carbonyls
Cobalt
Coordination Complexes - chemistry
Hydrogen-Ion Concentration
Metalorganic compounds
Myoglobin - chemistry
Organometallic Compounds - chemistry
Prints
Raw
Spectrophotometry, Ultraviolet - methods
Spectroscopy
Online AccessGet full text

Cover

More Information
Summary:The deoxy-myoglobin (deoxy-Mb)/carbonmonoxy-myoglobin (Mb-CO) UV-vis assay is the principal method used for quantifying the rates of CO release from CO-releasing molecules (CO-RMs) that might possess therapeutic benefits. Some issues emerge when the Mb-CO assay is utilized for testing CO-RMs with novel structures, which are comprehensively discussed here for the first time. Two methods for processing raw UV-vis spectroscopic data generated from the assay are presented in this paper.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:1477-9226
1477-9234
DOI:10.1039/c0dt01809k