The Altered Expression of microRNA408 Influences the Arabidopsis Response to Iron Deficiency

MicroRNAs contribute to the adaptation of plants to varying environmental conditions by affecting systemic mineral nutrient homeostasis. Copper and iron deficiencies antagonistically control the expression of ( ), which post-transcriptionally regulates laccase-like multicopper oxidase family members...

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Published inFrontiers in plant science Vol. 10; p. 324
Main Authors Carrió-Seguí, Àngela, Ruiz-Rivero, Omar, Villamayor-Belinchón, Laura, Puig, Sergi, Perea-García, Ana, Peñarrubia, Lola
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 02.04.2019
Subjects
Arabidopsis
hydrogen peroxide
iron deficiency
lignin
microRNA408
Plant Science
lignin
iron deficiency
Arabidopsis
microRNA408
hydrogen peroxide
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Summary:MicroRNAs contribute to the adaptation of plants to varying environmental conditions by affecting systemic mineral nutrient homeostasis. Copper and iron deficiencies antagonistically control the expression of ( ), which post-transcriptionally regulates laccase-like multicopper oxidase family members , , and . In this work, we used T-DNA insertion mutants (408-KO1 and 408-KO2) and a previously characterized transgenic line overexpressing (35S:408-14) to explore how influences copper- and iron-dependent metabolism. We observed that the altered expression of diminished plant performance and the activation of the iron-regulated genes under iron-deficient conditions. Consistently with the low expression of the -target laccases, we showed that the vascular bundle lignification of the plants diminished. The decrease in the phenoloxidase and ferroxidase activities exhibited by wild-type plants under iron deficiency did not occur in the 408-KO1 plants, probably due to the higher expression of laccases. Finally, we observed that the hydrogen peroxide levels under iron starvation were altered in both the and lines. Taken together, these results suggest that Arabidopsis plants with modified levels undergo multiple deregulations under iron-deficient conditions.
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Edited by: Wolfgang Schmidt, Academia Sinica, Taiwan
This article was submitted to Plant Nutrition, a section of the journal Frontiers in Plant Science
Present address: Àngela Carrió-Seguí, Instituto de Biología Molecular y Celular de Plantas (CSIC-Universidad Politécnica de Valencia), Valencia, Spain; Omar Ruiz-Rivero, Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Valencia, Spain; Laura Villamayor-Belinchón,Instituto de Medicina Genómica, Universitat de València, Valencia, Spain
Reviewed by: Gang Liang, Xishuangbanna Tropical Botanical Garden (CAS), China; Diqiu Yu, Xishuangbanna Tropical Botanical Garden (CAS), China; Rongrong Guo, Guangxi Academy of Agricultural Science, China
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2019.00324