Genome-Wide Identification of Dicer-Like, Argonaute, and RNA-Dependent RNA Polymerase Gene Families in Brassica Species and Functional Analyses of Their Arabidopsis Homologs in Resistance to Sclerotinia sclerotiorum
RNA silencing is an important mechanism to regulate gene expression and antiviral defense in plants. Nevertheless, RNA silencing machinery in the important oil crop and function in resistance to the devastating fungal pathogen are not well-understood. In this study, gene families of RNA silencing ma...
Saved in:
Published in | Frontiers in plant science Vol. 7; p. 1614 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
27.10.2016
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | RNA silencing is an important mechanism to regulate gene expression and antiviral defense in plants. Nevertheless, RNA silencing machinery in the important oil crop
and function in resistance to the devastating fungal pathogen
are not well-understood. In this study, gene families of RNA silencing machinery in
were identified and their role in resistance to
was revealed. Genome of the allopolyploid species
possessed 8
(
), 27
(
), and 16
(
) genes, which included almost all copies from its progenitor species
and
and three extra copies of
genes, indicating that the
group in
appears to have undergone further expansion through duplication during evolution. Moreover, compared with Arabidopsis, some
and
genes such as
, and
had significantly expanded in these
species. Twenty-one out of 51
, and
genes were predicted to contain calmodulin-binding transcription activators (CAMTA)-binding site (CGCG box).
inoculation strongly induced the expression of
genes while significantly suppressed that of some CGCG-containing RNA silencing component genes, suggesting that RNA silencing machinery might be targeted by CAMTA3. Furthermore, Arabidopsis mutant analyses demonstrated that
, and
mutants were more susceptible to
, while
was more resistant. Our results reveal the importance of RNA silencing in plant resistance to
and imply a new mechanism of CAMTA function as well as RNA silencing regulation. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Plant Biotic Interactions, a section of the journal Frontiers in Plant Science Edited by: Dirk Balmer, Syngenta Crop Protection, Switzerland Reviewed by: Biswapriya Biswavas Misra, Texas Biomedical Research Institute, USA; Airong Wang, Fujian Agriculture and Forestry University, China |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2016.01614 |