Development in primary cell culture of demosponges

• Published in: Journal of biotechnology Vol. 100; no. 2; pp. 119 - 125
• Main Authors: De Rosa, Salvatore, De Caro, Salvatore, Iodice, Carmine, Tommonaro, Giuseppina, Stefanov, Kamen, Popov, Simeon
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Lausanne Elsevier B.V 23.01.2003; Amsterdam Elsevier; New York, NY
• Subjects: Animals; aquaculture techniques; Biological and medical sciences; Biotechnology; Cell Division - drug effects; Cell Division - physiology; Cell Survival - drug effects; Cell Survival - physiology; Cells, Cultured; Cholesterol - pharmacology; Culture Media - pharmacology; Culture Techniques - methods; Demosponge; Demospongiae; Densitometry - methods; Dysidea avara; Equipment Contamination - prevention & control; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; Growth promotion; Hydrogen-Ion Concentration; Ircinia muscarum; Light; Lipid Metabolism; Marine; Photic Stimulation; Porifera - classification; Porifera - cytology; Porifera - microbiology; Porifera - physiology; Reference Values; Sensitivity and Specificity; Species Specificity; Sponge cell cultures; sponge culture; Suberites domuncula; Vitamin A - pharmacology; Ircinia muscarum; Biotechnology; Growth promotion; Sponge cell cultures; Dysidea avara; Suberites domuncula; Demosponge
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/S0168-1656(02)00252-3

We have established primary cell culture of the marine demosponge Dysidea avara and Suberites domuncula. Microbial contamination was controlled by the use of a pool of antibiotics confirming the goodness of this procedure. Effect of pH, temperature and light was studied to establish the better growth conditions. The comparison of lipid composition of sponge and cells suggested a series of experiments to optimise the medium. A glucose dose-dependent experiment showed that the ideal glucose concentration is 1 g l −1. Supplementing the medium with unsaturated fatty acid and retinol, no promotion of growth was observed, but the compounds were totally metabolised by cells. Increments from 70 to 160% in the number of cells were observed, supplementing the medium with different concentration of cholesterol. These results suggest that the analysis of the chemical composition of sponge and cells give indication on the composition of the nutrient media.