Mitogen-activated protein kinase signal transduction and DNA repair network are involved in aluminum-induced DNA damage and adaptive response in root cells of Allium cepa L
In the current study, we studied the role of signal transduction in aluminum (Al(3+))-induced DNA damage and adaptive response in root cells of Allium cepa L. The root cells in planta were treated with Al(3+) (800 μM) for 3 h without or with 2 h pre-treatment of inhibitors of mitogen-activated prote...
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Published in | Frontiers in plant science Vol. 5; p. 256 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
05.06.2014
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Subjects | |
Online Access | Get full text |
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Summary: | In the current study, we studied the role of signal transduction in aluminum (Al(3+))-induced DNA damage and adaptive response in root cells of Allium cepa L. The root cells in planta were treated with Al(3+) (800 μM) for 3 h without or with 2 h pre-treatment of inhibitors of mitogen-activated protein kinase (MAPK), and protein phosphatase. Also, root cells in planta were conditioned with Al(3+) (10 μM) for 2 h and then subjected to genotoxic challenge of ethyl methane sulfonate (EMS; 5 mM) for 3 h without or with the pre-treatment of the aforementioned inhibitors as well as the inhibitors of translation, transcription, DNA replication and repair. At the end of treatments, roots cells were assayed for cell death and/or DNA damage. The results revealed that Al(3+) (800 μM)-induced significant DNA damage and cell death. On the other hand, conditioning with low dose of Al(3+) induced adaptive response conferring protection of root cells from genotoxic stress caused by EMS-challenge. Pre-treatment of roots cells with the chosen inhibitors prior to Al(3+)-conditioning prevented or reduced the adaptive response to EMS genotoxicity. The results of this study suggested the involvement of MAPK and DNA repair network underlying Al-induced DNA damage and adaptive response to genotoxic stress in root cells of A. cepa. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Reinhard Kunze, Freie Universitat Berlin, Germany; Alma Balestrazzi, University of Pavia, Italy; Mattia Donà, Gregor Mendel Institute of Plant Molecular Biology, Austria This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science. Edited by: Alma Balestrazzi, University of Pavia, Italy Present address: V. Mohan M. Achary, Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2014.00256 |