Association between DNA variant sites in the apolipoprotein A5 gene and coronary heart disease in Chinese

• Published in: Metabolism, clinical and experimental Vol. 54; no. 5; pp. 568 - 572
• Main Authors: Liu, Hekun, Zhang, Sizhong, Lin, Jianyin, Li, Hai, Huang, Aimin, Xiao, Cuiying, Li, Xuefei, Su, Zhiguang, Wang, Chunting, Nebert, Daniel W., Zhou, Bing, Zheng, Keqin, Shi, Jiajun, Li, Guixin, Huang, Dejia
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.05.2005; Elsevier
• Subjects: Apolipoprotein A-V; Apolipoprotein A5; Apolipoproteins - genetics; Apolipoproteins A; Asian Continental Ancestry Group - genetics; Atherosclerosis (general aspects, experimental research); Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Case-Control Studies; Chinese population study; Cholesterol, HDL - blood; Coronary Disease - blood; Coronary Disease - genetics; Coronary heart disease; Cytosine; DNA - genetics; Fasting - blood; Female; Genetic Variation; Heart; Heterozygote; Humans; Male; Medical sciences; Middle Aged; Polymorphism, Single Nucleotide; Serine; Single nucleotide polymorphisms; Thymine; Triglycerides; Triglycerides - blood; Tryptophan; Triglycerides; Single nucleotide polymorphisms; Apolipoprotein A5; Chinese population study; Coronary heart disease; Human; Site; Lipids; Cardiovascular disease; Patient; Apolipoprotein; Triglyceride; Frequency control; Blood plasma; Vascular disease; Polymerase chain reaction; Variant; Association; Restriction fragment length polymorphism; Gene; DNA; Atherosclerosis; Risk factor; Total; Chinese; Population; Level
• ISSN: 0026-0495; 1532-8600
• DOI: 10.1016/j.metabol.2004.11.009

The recently discovered apolipoprotein A5 ( APOA5) gene has been shown to be important in determining plasma triglyceride levels, a major cardiovascular disease risk factor. We searched for possible associations of the APOA5 gene polymorphisms S19W and −1131T>C with coronary heart disease (CHD) in a Chinese population. A total of 483 Chinese CHD patients and 502 control non-CHD subjects were genotyped by polymerase chain reaction–restriction fragment length polymorphism for these 2 single nucleotide polymorphisms. We found that the minor allele 19W was observed only in CHD patients and not in controls, with allelic frequencies of 0.047 and 0.000, respectively ( P < .000001), and the minor allele −1131C was significantly higher in CHD patients than in controls (0.391 vs 0.299, P < .0001). These results suggest that both the S19W and −1131T>C variations in the APOA5 gene are associated with the CHD and appear to be 2 genetic risk factors for CHD susceptibility in Chinese. Moreover, we found that triglyceride levels were significantly higher in −1131C carriers than in −1131T subjects of the control group and that high-density–lipoprotein cholesterol was decreased in −1131C carriers among CHD patients.