GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis
Plant WRKY transcription factors (TFs) are active guardians against pathogens' insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functiona...
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| Published in | Frontiers in plant science Vol. 9; p. 809 |
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| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
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Frontiers Media S.A
26.06.2018
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| Abstract | Plant WRKY transcription factors (TFs) are active guardians against pathogens' insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally characterized a new WRKY TF GmWRKY49 from soybean. GmWRKY49 is a nuclear protein which contains two highly conserved WRKY domains and a C
H
-type zinc-finger structure. The normalized expression (log
ratio) of
was 2.75- and 1.90-fold in salt-tolerant and salt-susceptible soybean genotypes, respectively. The transcripts of
could be detected in roots, stems, leaves, flowers, and almost no expression in pod tissues. The salinity-tolerance response of this gene was studied through overexpression in soybean composite seedlings and transgenic
. The effect of
overexpression on root length of transgenic
was also investigated. Under salt stress, several parameters including germination rate, survival rate, root length, rosette diameter, relative electrolyte leakage, and proline content were significantly higher in composite seedlings and transgenic
than those in wild-type. Moreover,
enhanced salinity tolerance in soybean mosaic seedlings and transgenic
. These results suggest that
is a positive regulator of salinity tolerance in soybean and has high potential utilization for crop improvement. |
|---|---|
| AbstractList | Plant WRKY transcription factors (TFs) are active guardians against pathogens’ insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally characterized a new WRKY TF GmWRKY49 from soybean. GmWRKY49 is a nuclear protein which contains two highly conserved WRKY domains and a C2H2-type zinc-finger structure. The normalized expression (log2 ratio) of GmWRKY49 was 2.75- and 1.90-fold in salt-tolerant and salt-susceptible soybean genotypes, respectively. The transcripts of GmWRKY49 could be detected in roots, stems, leaves, flowers, and almost no expression in pod tissues. The salinity-tolerance response of this gene was studied through overexpression in soybean composite seedlings and transgenic Arabidopsis. The effect of GmWRKY49 overexpression on root length of transgenic Arabidopsis was also investigated. Under salt stress, several parameters including germination rate, survival rate, root length, rosette diameter, relative electrolyte leakage, and proline content were significantly higher in composite seedlings and transgenic Arabidopsis than those in wild-type. Moreover, GmWRKY49 enhanced salinity tolerance in soybean mosaic seedlings and transgenic Arabidopsis. These results suggest that GmWRKY49 is a positive regulator of salinity tolerance in soybean and has high potential utilization for crop improvement. Plant WRKY transcription factors (TFs) are active guardians against pathogens' insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally characterized a new WRKY TF GmWRKY49 from soybean. GmWRKY49 is a nuclear protein which contains two highly conserved WRKY domains and a C H -type zinc-finger structure. The normalized expression (log ratio) of was 2.75- and 1.90-fold in salt-tolerant and salt-susceptible soybean genotypes, respectively. The transcripts of could be detected in roots, stems, leaves, flowers, and almost no expression in pod tissues. The salinity-tolerance response of this gene was studied through overexpression in soybean composite seedlings and transgenic . The effect of overexpression on root length of transgenic was also investigated. Under salt stress, several parameters including germination rate, survival rate, root length, rosette diameter, relative electrolyte leakage, and proline content were significantly higher in composite seedlings and transgenic than those in wild-type. Moreover, enhanced salinity tolerance in soybean mosaic seedlings and transgenic . These results suggest that is a positive regulator of salinity tolerance in soybean and has high potential utilization for crop improvement. Plant WRKY transcription factors (TFs) are active guardians against pathogens' insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally characterized a new WRKY TF GmWRKY49 from soybean. GmWRKY49 is a nuclear protein which contains two highly conserved WRKY domains and a C2H2-type zinc-finger structure. The normalized expression (log2 ratio) of GmWRKY49 was 2.75- and 1.90-fold in salt-tolerant and salt-susceptible soybean genotypes, respectively. The transcripts of GmWRKY49 could be detected in roots, stems, leaves, flowers, and almost no expression in pod tissues. The salinity-tolerance response of this gene was studied through overexpression in soybean composite seedlings and transgenic Arabidopsis. The effect of GmWRKY49 overexpression on root length of transgenic Arabidopsis was also investigated. Under salt stress, several parameters including germination rate, survival rate, root length, rosette diameter, relative electrolyte leakage, and proline content were significantly higher in composite seedlings and transgenic Arabidopsis than those in wild-type. Moreover, GmWRKY49 enhanced salinity tolerance in soybean mosaic seedlings and transgenic Arabidopsis. These results suggest that GmWRKY49 is a positive regulator of salinity tolerance in soybean and has high potential utilization for crop improvement.Plant WRKY transcription factors (TFs) are active guardians against pathogens' insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally characterized a new WRKY TF GmWRKY49 from soybean. GmWRKY49 is a nuclear protein which contains two highly conserved WRKY domains and a C2H2-type zinc-finger structure. The normalized expression (log2 ratio) of GmWRKY49 was 2.75- and 1.90-fold in salt-tolerant and salt-susceptible soybean genotypes, respectively. The transcripts of GmWRKY49 could be detected in roots, stems, leaves, flowers, and almost no expression in pod tissues. The salinity-tolerance response of this gene was studied through overexpression in soybean composite seedlings and transgenic Arabidopsis. The effect of GmWRKY49 overexpression on root length of transgenic Arabidopsis was also investigated. Under salt stress, several parameters including germination rate, survival rate, root length, rosette diameter, relative electrolyte leakage, and proline content were significantly higher in composite seedlings and transgenic Arabidopsis than those in wild-type. Moreover, GmWRKY49 enhanced salinity tolerance in soybean mosaic seedlings and transgenic Arabidopsis. These results suggest that GmWRKY49 is a positive regulator of salinity tolerance in soybean and has high potential utilization for crop improvement. Plant WRKY transcription factors (TFs) are active guardians against pathogens’ insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally characterized a new WRKY TF GmWRKY49 from soybean. GmWRKY49 is a nuclear protein which contains two highly conserved WRKY domains and a C 2 H 2 -type zinc-finger structure. The normalized expression (log 2 ratio) of GmWRKY49 was 2.75- and 1.90-fold in salt-tolerant and salt-susceptible soybean genotypes, respectively. The transcripts of GmWRKY49 could be detected in roots, stems, leaves, flowers, and almost no expression in pod tissues. The salinity-tolerance response of this gene was studied through overexpression in soybean composite seedlings and transgenic Arabidopsis . The effect of GmWRKY49 overexpression on root length of transgenic Arabidopsis was also investigated. Under salt stress, several parameters including germination rate, survival rate, root length, rosette diameter, relative electrolyte leakage, and proline content were significantly higher in composite seedlings and transgenic Arabidopsis than those in wild-type. Moreover, GmWRKY49 enhanced salinity tolerance in soybean mosaic seedlings and transgenic Arabidopsis . These results suggest that GmWRKY49 is a positive regulator of salinity tolerance in soybean and has high potential utilization for crop improvement. |
| Author | Zhang, Dayong Ma, Hongxiang Xu, Zhaolong Yi, Jinxin Shao, Hong-Bo Raza, Qasim He, Xiaolan Xu, Ling Huang, Yihong Ali, Zulfiqar |
| AuthorAffiliation | 4 Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Yancheng Teachers University , Yancheng , China 5 Institute of Grain Crops, Jiangsu Academy of Agricultural Sciences , Nanjing , China 1 Salt-soil Agricultural Center, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences , Nanjing , China 2 Department of Plant Breeding and Genetics, Muhammad Nawaz Shareef University of Agriculture , Multan , Pakistan 3 Rice Research Institute , Kala Shah Kaku , Pakistan |
| AuthorAffiliation_xml | – name: 1 Salt-soil Agricultural Center, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences , Nanjing , China – name: 2 Department of Plant Breeding and Genetics, Muhammad Nawaz Shareef University of Agriculture , Multan , Pakistan – name: 3 Rice Research Institute , Kala Shah Kaku , Pakistan – name: 5 Institute of Grain Crops, Jiangsu Academy of Agricultural Sciences , Nanjing , China – name: 4 Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Yancheng Teachers University , Yancheng , China |
| Author_xml | – sequence: 1 givenname: Zhaolong surname: Xu fullname: Xu, Zhaolong – sequence: 2 givenname: Qasim surname: Raza fullname: Raza, Qasim – sequence: 3 givenname: Ling surname: Xu fullname: Xu, Ling – sequence: 4 givenname: Xiaolan surname: He fullname: He, Xiaolan – sequence: 5 givenname: Yihong surname: Huang fullname: Huang, Yihong – sequence: 6 givenname: Jinxin surname: Yi fullname: Yi, Jinxin – sequence: 7 givenname: Dayong surname: Zhang fullname: Zhang, Dayong – sequence: 8 givenname: Hong-Bo surname: Shao fullname: Shao, Hong-Bo – sequence: 9 givenname: Hongxiang surname: Ma fullname: Ma, Hongxiang – sequence: 10 givenname: Zulfiqar surname: Ali fullname: Ali, Zulfiqar |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/29997634$$D View this record in MEDLINE/PubMed |
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| ContentType | Journal Article |
| Copyright | Copyright © 2018 Xu, Raza, Xu, He, Huang, Yi, Zhang, Shao, Ma and Ali. 2018 Xu, Raza, Xu, He, Huang, Yi, Zhang, Shao, Ma and Ali |
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| Keywords | salinity tolerance GmWRKY49 overexpression WRKY TFs Glycine max |
| Language | English |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Truyen Quach, University of Nebraska–Lincoln, United States; Xiaorong Fan, Nanjing Agricultural University, China Edited by: Lam-Son Tran, RIKEN, Japan These authors have contributed equally to this work. This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science |
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| Title | GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis |
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