GmWRKY49, a Salt-Responsive Nuclear Protein, Improved Root Length and Governed Better Salinity Tolerance in Transgenic Arabidopsis

Plant WRKY transcription factors (TFs) are active guardians against pathogens' insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functiona...

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Published inFrontiers in plant science Vol. 9; p. 809
Main Authors Xu, Zhaolong, Raza, Qasim, Xu, Ling, He, Xiaolan, Huang, Yihong, Yi, Jinxin, Zhang, Dayong, Shao, Hong-Bo, Ma, Hongxiang, Ali, Zulfiqar
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 26.06.2018
Subjects
Glycine max
GmWRKY49
overexpression
Plant Science
salinity tolerance
WRKY TFs
salinity tolerance
GmWRKY49
overexpression
WRKY TFs
Glycine max
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Summary:Plant WRKY transcription factors (TFs) are active guardians against pathogens' insurgency, key components in developmental processes, contributors in signal transduction pathways, and regulators of diverse biotic and abiotic stress responses. In this research, we isolated, cloned, and functionally characterized a new WRKY TF GmWRKY49 from soybean. GmWRKY49 is a nuclear protein which contains two highly conserved WRKY domains and a C H -type zinc-finger structure. The normalized expression (log ratio) of was 2.75- and 1.90-fold in salt-tolerant and salt-susceptible soybean genotypes, respectively. The transcripts of could be detected in roots, stems, leaves, flowers, and almost no expression in pod tissues. The salinity-tolerance response of this gene was studied through overexpression in soybean composite seedlings and transgenic . The effect of overexpression on root length of transgenic was also investigated. Under salt stress, several parameters including germination rate, survival rate, root length, rosette diameter, relative electrolyte leakage, and proline content were significantly higher in composite seedlings and transgenic than those in wild-type. Moreover, enhanced salinity tolerance in soybean mosaic seedlings and transgenic . These results suggest that is a positive regulator of salinity tolerance in soybean and has high potential utilization for crop improvement.
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Reviewed by: Truyen Quach, University of Nebraska–Lincoln, United States; Xiaorong Fan, Nanjing Agricultural University, China
Edited by: Lam-Son Tran, RIKEN, Japan
These authors have contributed equally to this work.
This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2018.00809