Light Control of Salt-Induced Proline Accumulation Is Mediated by ELONGATED HYPOCOTYL 5 in Arabidopsis

• Published in: Frontiers in plant science Vol. 10; p. 1584
• Main Authors: Kovács, Hajnalka, Aleksza, Dávid, Baba, Abu Imran, Hajdu, Anita, Király, Anna Mária, Zsigmond, Laura, Tóth, Szilvia Z, Kozma-Bognár, László, Szabados, László
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 10.12.2019
• Subjects: Arabidopsis; ELONGATED HYPOCOTYL 5; gene expression regulation; light signalling; Plant Science; proline accumulation; gene expression regulation; proline accumulation; ELONGATED HYPOCOTYL 5; light signalling; Arabidopsis
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2019.01584

Plants have to adapt their metabolism to constantly changing environmental conditions, among which the availability of light and water is crucial in determining growth and development. Proline accumulation is one of the sensitive metabolic responses to extreme conditions; it is triggered by salinity or drought and is regulated by light. Here we show that red and blue but not far-red light is essential for salt-induced proline accumulation, upregulation of ( ) and downregulation of ( ) genes, which control proline biosynthetic and catabolic pathways, respectively. Chromatin immunoprecipitation and electrophoretic mobility shift assays demonstrated that the transcription factor ELONGATED HYPOCOTYL 5 (HY5) binds to G-box and C-box elements of and a C-box motif of . Salt-induced proline accumulation and expression were reduced in the double mutant, suggesting that HY5 promotes proline biosynthesis through connecting light and stress signals. Our results improve our understanding on interactions between stress and light signals, confirming HY5 as a key regulator in proline metabolism.