Differential proteomics of Aedes albopictus salivary gland, midgut and C6/36 cell induced by dengue virus infection

• Published in: Virology (New York, N.Y.) Vol. 444; no. 1; pp. 109 - 118
• Main Authors: Zhang, Meichun, Zheng, Xiaoying, Wu, Yu, Gan, Ming, He, Ai, Li, Zhuoya, Zhang, Dongjing, Wu, Xiansheng, Zhan, Ximei
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2013
• Subjects: Aedes - chemistry; Aedes albopictus; Animals; Cell Line; Dengue virus; Dengue Virus - pathogenicity; Differential proteomics; Electrophoresis, Gel, Two-Dimensional; Gastrointestinal Tract - chemistry; Gene Expression Profiling; Host-Pathogen Interactions; Infectious Disease; Mass Spectrometry; Proteome - analysis; Real-Time Polymerase Chain Reaction; RNA, Messenger - analysis; RNA, Messenger - genetics; Salivary Glands - chemistry; Dengue virus; Differential proteomics; Aedes albopictus
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/j.virol.2013.06.001

Abstract The interaction between dengue virus (DENV) and vector mosquitoes are still poorly understood at present. In this study, 2-D DIGE combined with MS was used to analyze the differential proteomes of Aedes albopictus salivary gland, midgut and C6/36 cells induced by DENV-2. Our results indicated that the virus infection regulated several functional classes of proteins. Among them, 26 were successfully analyzed by real-time RT-PCR. The mRNA levels of 15 were the highest in salivary gland, 2 in midgut and none in C6/36 cells, however, 18 were the least in fat body compared to other organs. Interestingly, the changes of differential proteins mRNA were the most obvious in fat body post-infection. Chaperone, cytoskeleton and energy metabolism enzyme were the most down- or up- regulated proteins after DENV-2 infection. The abundant expression of these proteins in salivary gland may relate to its high susceptibility.