Differential gene modulation of pattern-recognition receptor TLR and RIG-I-like and downstream mediators on intestinal mucosa of pigs infected with PEDV non S-INDEL and PEDV S-INDEL strains
Porcine epidemic diarrhea virus (PEDV) strains can be divided into non-S-INDEL and S-INDEL strains. PEDV pathogenesis is strain-specific, and studies in neonatal pigs have demonstrated that the PEDV non-S-INDEL strains are more pathogenic than the PEDV S-INDEL strains. RNA viruses, including PEDV, c...
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Published in | Virology (New York, N.Y.) Vol. 517; pp. 188 - 198 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.04.2018
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Subjects | |
Online Access | Get full text |
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Summary: | Porcine epidemic diarrhea virus (PEDV) strains can be divided into non-S-INDEL and S-INDEL strains. PEDV pathogenesis is strain-specific, and studies in neonatal pigs have demonstrated that the PEDV non-S-INDEL strains are more pathogenic than the PEDV S-INDEL strains. RNA viruses, including PEDV, can interact with a large number of pattern recognition receptors (PRRs) in the intestinal mucosa, including toll-like receptors (TLRs) and RIG-I-like receptors (RLRs). We investigated the differential gene modulation of TLRs, RIG-I, and downstream mediators on the intestinal mucosa of neonatal pigs infected with PEDV S-INDEL and non-S-INDEL strains. Ten five-day-old piglets were inoculated orally with 10ml of 104 TCDI50/ml of either PEDV non-S-INDEL or S-INDEL strains. PEDV S-INDEL infection induced pro-inflammatory cytokines through the non-canonical NF-κB signaling pathway by activating RIG-I. In contrast, PEDV non-S-INDEL infection suppressed the induction of pro-inflammatory cytokines and type 1 interferon production by down-regulation of TLRs and downstream signaling molecules.
•Differential gene modulation of TLR and RIG-I-like receptors and downstream mediators.•PEDV S-INDEL induces pro-inflammatory cytokines through non-canonical NF-κB signaling pathway.•PEDV S-INDEL pro-inflammatory cytokines activation is RIG-I dependent.•PEDV non-S-INDEL suppresses the induction of pro-inflammatory cytokines and type 1 interferon.•PEDV non-S-INDEL effect is mediated by down-regulation of TLRs and its downstream-signaling molecules.•PEDV S-INDEL and PEDV non-S-INDEL cause differential modulation on innate immune response pathways.•Differential modulation could be translated into differences in pathogenesis and clinical outcomes. |
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Bibliography: | These authors contributed equally to this work. |
ISSN: | 0042-6822 1096-0341 |
DOI: | 10.1016/j.virol.2017.11.024 |