Neonatal Reference Intervals for the Complete Blood Count Parameters MicroR and HYPO-He: Sensitivity Beyond the Red Cell Indices for Identifying Microcytic and Hypochromic Disorders

• Published in: The Journal of pediatrics Vol. 239; pp. 95 - 100.e2
• Main Authors: Bahr, Timothy M., Christensen, Thomas R., Henry, Erick, Wilkes, Jacob, Ohls, Robin K., Bennett, Sterling T., Ward, Diane M., Pysher, Theodore J., Christensen, Robert D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2021
• Subjects: Anemia, Iron-Deficiency - blood; Anemia, Iron-Deficiency - diagnosis; Biomarkers - blood; Humans; hypochromia; Infant; Infant, Newborn; iron deficiency; MCH; MCV; microcytosis; reference intervals; Reference Values; Reticulocyte Count - methods; Reticulocytes - chemistry; Retrospective Studies; MCV; RBC; MCH; microcytosis; CBC; RET-He; reference intervals; iron deficiency; hypochromia
• ISSN: 0022-3476; 1097-6833
• DOI: 10.1016/j.jpeds.2021.08.002

To create neonatal reference intervals for the MicroR and HYPO-He complete blood count (CBC) parameters and to test whether these parameters are sensitive early markers of disease at early stages of microcytic/hypochromic disorders while the CBC indices are still normal. We retrospectively collected the CBC parameters MicroR and HYPO-He, along with the standard CBC parameters, from infants aged 0-90 days at Intermountain Healthcare hospitals using Sysmex hematology analyzers. We created reference intervals for these parameters by excluding values from neonates with proven microcytic disorders (ie, iron deficiency or alpha thalassemia) from the dataset. From >11 000 CBCs analyzed, we created reference intervals for MicroR and HYPO-He in neonates aged 0-90 days. The upper intervals are considerably higher in neonates than in adults, validating increased anisocytosis and polychromasia among neonates. Overall, 52% of neonates with iron deficiency (defined by reticulocyte hemoglobin equivalent <25 pg) had a MicroR >90% upper interval (relative risk, 4.14; 95% CI, 3.80-4.53; P < .001), and 68% had an HYPO-He >90% upper interval (relative risk, 6.64; 95% CI, 6.03-7.32; P < .001). These 2 new parameters were more sensitive than the red blood cell (RBC) indices (P < .001) in identifying 24 neonates with iron deficiency at birth. We created neonatal reference intervals for MicroR and HYPO-He. Although Sysmex currently designates these as research use only in the US, they can be measured as part of a neonate’s CBC with no additional phlebotomy volume or run time and can identify microcytic and hypochromic disorders even when the RBC indices are normal.