MeABL5 , an ABA Insensitive 5-Like Basic Leucine Zipper Transcription Factor, Positively Regulates MeCWINV3 in Cassava ( Manihot esculenta Crantz )
The basic leucine zipper (bZIP) transcription factor family plays crucial roles in multiple biological processes, especially stress responses. Cassava ( Crantz) is an important tropical crop with a strong tolerance to environmental stresses such as drought, heat, and low-fertility environments. Curr...
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Published in | Frontiers in plant science Vol. 10; p. 772 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
28.06.2019
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Subjects | |
Online Access | Get full text |
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Summary: | The basic leucine zipper (bZIP) transcription factor family plays crucial roles in multiple biological processes, especially stress responses. Cassava (
Crantz) is an important tropical crop with a strong tolerance to environmental stresses such as drought, heat, and low-fertility environments. Currently, limited information is available regarding the functional identification of bZIP transcription factors in response to abiotic stress in cassava. Herein, a gene encoding an ABA Insensitive 5 (ABI5)-like transcription factor, designated as
, was identified in cassava. Sequence and phylogenetic analysis showed that MeABL5 is a cassava bZIP transcription factor that is not included in the previously identified cassava bZIP family members, belongs to subfamily A, and has high sequence similarity to ABI5-like proteins. Subcellular localization and transactivation assays revealed that MeABL5 was a nuclear-localized protein and possessed transactivation activity. Furthermore, MeABL5 was able to specifically interact with the ABRE cis-element in the promoter of the cassava major cell wall invertase gene,
and
and
exhibited similar expression patterns in various organs or tissues and under abiotic stress in cassava. The expressions of
and
within cassava plantlets were both induced by exogenous abscisic acid (ABA), gibberellic acid (GA3), methyl jasmonate (MeJA), and heat. Overexpression of
increased the activity of the
gene, and the up-regulated expressions of
were significantly activated under ABA-, salicylic acid (SA)-, and MeJA-induced conditions. Overall, these results suggest that
is a positive regulator of
and might participate in the robust resistance of cassava in response to abiotic stress. This study also provides a foundation for further research on ABA-mediated and stress-related signaling pathways in cassava. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Ning Tang, UMR5004 Biochimie et Physiologie Moléculaire des Plantes (BPMP), France; Jong-Seong Jeon, Kyung Hee University, South Korea Edited by: Ruth Grene, Virginia Tech, United States These authors have contributed equally to this work This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2019.00772 |